Size  100ug
Cat code  200493-2B5
Species Cross-reactivity  H,Mk
Key Application  WB,ICC
Host  Mouse
Antibody type  Monoclonal antibody
Purified method  Affinity purified
Isotype  IgG2a
Molecular Weight  46kDa
Sensitivity  This antibody detects endogenous levels of krt18 and does not cross-react with related proteins.
Immunogen  Purified recombinant human KRT18 protein fragments expressed in E.coli.
UniProt Number  P05783
GeneBank ID  NM_000224
Subcellular location  Cytoplasm ? perinuclear region
Formulation  Purified mouse monoclonal in PBS(pH 7.4) containing with 0.02% sodium azide,0.1mg/mlBSA and 50% glycerol.
Altername  Cell proliferation-inducing gene 46 protein;Cytokeratin-18;Keratin-18;krt18;
Gene_symbol  K18;CYK18.
Summary  KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene.
UniPort summary  Function  Involved in the uptake of thrombin-antithrombin complexes by hepatic cells By similarity. When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
Application Images :  
Western blot detection of krt18 antibody in Hela,COS7 and 293T cell lysates using krt18 antibody (1:2000 diluted).Predicted band size:46KDa.Observed band size:46KDa.
Immunocytochemistry staining of HeLa cells fixed by anhydrous methanol for 2 h at -20℃ and using anti-krt18 antibody (dilution 1:800).