Size  100ug
Cat code  200605
Species Cross-reactivity  H,R
Key Application  WB,ICC
Host  Mouse
Antibody type  Monoclonal antibody
Purified method  Affinity purified
Isotype  IgG2b
Molecular Weight  50kDa
Sensitivity  This antibody detects endogenous levels of CSK and does not cross-react with related proteins.
Immunogen  Purified recombinant human CSK protein fragments expressed in E.coli.
UniProt Number  P41240
GeneBank ID  NM_001127190
Subcellular location  Cytoplasm. Cell membrane.
Formulation  Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine(pH 7.4,150 mM NaCl)with 0.2% sodium azide,0.1mg/mlBSA and 50% glycerol.
Altername  C SRC;C SRC kinase;C src Tyrosine Kinase;C-SRC kinase;c-src tyrosine kinase;Csk A;CSK;CSK_HUMAN;CYTOPLASMIC TYROSINE KINASE;EC2.7.10.2;MGC112926;MGC117393;MGC154049;P60 Src;Protein tyrosine kinase CYL;Protein-tyrosine kinase CYL;Proto oncogene tyrosine protein kinase;Tyrosine protein kinase CSK;Tyrosine-protein kinase CSK;zgc:154049.
Gene_symbol  null
Summary  null
UniPort summary  Function  Non-receptor tyrosine-protein kinase that plays an important role in the regulation of cell growth, differentiation, migration and immune response. Phosphorylates tyrosine residues located in the C-terminal tails of Src-family kinases (SFKs) including LCK, SRC, HCK, FYN, LYN or YES1. Upon tail phosphorylation, Src-family members engage in intramolecular interactions between the phosphotyrosine tail and the SH2 domain that result in an inactive conformation. To inhibit SFKs, CSK is recruited to the plasma membrane via binding to transmembrane proteins or adapter proteins located near the plasma membrane. Suppresses signaling by various surface receptors, including T-cell receptor (TCR) and B-cell receptor (BCR) by phosphorylating and maintaining inactive several positive effectors such as FYN or LCK.
Application Images :  
Western blot detection of CSK antibody in C6 and Jurkat cell lysates using CSK antibody (1:1000 diluted).Predicted band size:50KDa.Observed band size:50KDa.
Immunocytochemistry staining of C6 cells fixed by anhydrous methanol for 2 h at -20℃ and using anti-CSK antibody (dilution 1:50).