Anti-MLH1-抗体-抗体-生物在线
Anti-MLH1

Anti-MLH1

商家询价

产品名称: Anti-MLH1

英文名称:

产品编号: 200956

产品价格: null

产品产地: 成都正能生物

品牌商标: Zen BioScience

更新时间: null

使用范围: WB

成都正能生物技术有限责任公司
  • 联系人 :
  • 地址 : 中国成都高新区科园南路爱斯特大厦
  • 邮编 : 610200
  • 所在区域 : 四川
  • 电话 : 180****2350
  • 传真 : +86-028-85739596
  • 邮箱 : sales@zen-bioscience.com

Size  100ug

Cat code  200956

Species Cross-reactivity  H

Key Application  WB

Host  Mouse

Antibody type  Monoclonal antibody

Purified method  Affinity purified

Isotype  IgG2b

Molecular Weight  85kDa

Sensitivity  This antibody detects endogenous levels of MLH1 and does not cross-react with related proteins.

Immunogen  Purified recombinant human MLH1 protein fragments expressed in E.coli.

UniProt Number  P40692

GeneBank ID  NM_000249

Subcellular location  Nucleus

Formulation  Purified mouse monoclonal in PBS(pH 7.4) containing with 0.02% sodium azide,0.1mg/mlBSA and 50% glycerol.

Altername  COCA 2;COCA2;DNA mismatch repair protein Mlh1;FCC 2;FCC2;hMLH 1;hMLH1;HNPCC 2;HNPCC;HNPCC2;MGC5172;MLH 1;MLH1;MLH1_HUMAN;MutL homolog 1 (E. coli);MutL homolog 1;MutL homolog 1 colon cancer nonpolyposis type 2;MutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli);MutL protein homolog 1;MutL, E. coli, homolog of, 1.

Gene_symbol  FCC2;COCA2;HNPCC;hMLH1;HNPCC2.

Summary  This gene was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC).It is a human homolog of the E. coli DNA mismatch repair gene mutL,consistent with the characteristic alterations in microsatellite sequences (RER+phenotype) found in HNPCC.Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described,but their full-length natures have not been determined.[provided by RefSeq,Nov 2009]

UniPort summary Function  Heterodimerizes with PMS2 to form MutL alpha,a component of the post-replicative DNA mismatch repair system (MMR).DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH6) binding to a dsDNA mismatch,then MutL alpha is recruited to the heteroduplex.Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2.It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch.DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected.MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III,suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR.Also implicated in DNA damage signaling,a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages.Heterodimerizes with MLH3 to form MutL gamma which plays a role in meiosis.

Application Images :  

Western blot detection of MLH1 antibody in Hela,293T and Jurkat cell lysates using MLH1 antibody (1:500 diluted).Predicted band size:85KDa.Observed band size:85KDa.