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14-3-3 epsilon抗体

14-3-3 epsilon抗体

商家询价

产品名称： 14-3-3 epsilon抗体

英文名称： 14-3-3 epsilonAntibody

产品编号： XY11648-2

产品价格： null

产品产地： 中国/美国

品牌商标： 信裕生物

更新时间： 2023-08-17T09:55:27

使用范围： WB,ELISA,IHC-P,IHC-F,IF

上海信裕生物科技有限公司
  • 联系人 : 徐经理
  • 地址 : 上海市闵行莘庄工业区春东路508号A1-2F
  • 邮编 : 200612
  • 所在区域 : 上海
  • 电话 : 152****8802
  • 传真 : 021-37680378
  • 邮箱 : shxysw02@163.com
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14-3-3 epsilon抗体

 Applications

In-house applications ELISA, WB, IHC, IF, IP, FC     Application not tested? Please see our Guarantee.
Species specificity Human,Mouse,Rat; other species not tested.
Images
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  • 11648-2-AP;A375 cellsA375 cells were subjected to SDS PAGE followed by western blot with 11648-2-AP(14-3-3E antibody) at dilution of 1:500
  • 11648-2-AP;A375 cellsIP Result of anti-14-3-3E (IP:11648-2-AP, 3ug; Detection:11648-2-AP 1:1000) with A375 cells lysate 6000ug.
  • 11648-2-AP;human gliomasImmunohistochemical of paraffin-embedded human gliomas using 11648-2-AP(14-3-3E antibody) at dilution of 1:100 (under 10x lens)
  • 11648-2-AP;HepG2Immunofluorescent analysis of HepG2 cells, using YWHAE antibody 11648-2-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
  • 11648-2-AP;HepG2 cells1X10^6 HepG2 cells were stained with 0.2ug 14-3-3E antibody (11648-2-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
Positive WB detected in A375 cells, HeLa cells, human heart tissue
Positive IP detected in A375 cells
Positive IHC detected in Human gliomas tissue
Positive IF detected in HepG2 cells
Positive FC detected in HepG2 cells
Recommended dilution WB : 1:1000-1:10000
IP : 1:500-1:5000
IHC : 1:20-1:200
IF : 1:10-1:100
14-3-3 epsilon抗体Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
Product Information
Source Rabbit Purification method Antigen affinity purification
Isotype IgG Storage PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC.
Immunogen Information
Immunogen 14-3-3 epsilon fusion protein ag2247 Full name Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide
Calculated molecular
weight		 255aa,29 kDa Observed molecular 
weight		 29 kDa
GenBank accession 
number		 BC000179 Gene ID (NCBI) 7531
Gene symbol YWHAE Synonyms 14 3 3 protein epsilon, 14 3 3E, 14-3-3, 14-3-3 epsilon, FLJ45465, KCIP 1, MDCR, MDS, YWHAE
Background

14-3-3 epsilon抗体14-3-3 epsilon (also known as YWHAE) is a member of 14-3-3 proteins which were the first phosphoserine/phosphothreonine-binding proteins to be discovered. 14-3-3 family members interact with a wide spectrum of proteins and possess diverse functions. Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers. 14-3-3 proteins display the highest expression levels in the brain, and have been implicated in several neurodegenerative diseases, including Alzheimer's disease and amyotrophic lateral sclerosis. This antibody was raised against full-length 14-3-314-3-3 epsilon抗体 epsilon.