CCL19,巨噬细胞炎性蛋白19抗体

产品编号HZ-2454R
英文名称CCL19
中文名称巨噬细胞炎性蛋白19抗体
别 名Macrophage Inflammatory Protein 3β; ;Macrophage Inflammatory Protein 3 beta; Beta chemokine exodus 3; C C chemokine ligand 19; CC chemokine ligand 19; CCL19; Chemokine (C C motif) ligand 19; Chemokine (CC motif) ligand 19; Chemokine CC Motif Ligand 19; CK beta 11; CKb11; EBI1 ligand chemokine; ELC; Exodus 3; MGC34433; MIP 3 beta; MIP3 beta; MIP3B; OTTHUMP00000000531; SCYA19; Small inducible cytokine A19; CCL19_MOUSE.
说 明 书0.1ml 0.2ml
研究领域细胞生物 免疫学 自然杀伤细胞
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Mouse, Rat, Pig,
CCL19,巨噬细胞炎性蛋白19抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量9.2kDa
细胞定位细胞外基质
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from mouse CCL19
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
CCL19,巨噬细胞炎性蛋白19抗体PubMedPubMed
产品介绍background:
This gene is one of several CC cytokine genes clustered on the p-arm of chromosome 9. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene may play a role in normal lymphocyte recirculation and homing. It also plays an important role in trafficking of T cells in thymus, and in T cell and B cell migration to secondary lymphoid organs. It specifically binds to chemokine receptor CCR7. [provided by RefSeq, Jul 2008]

Function:
Strongly chemotactic for naive (L-selectinhi) CD4 T-cells and for CD8 T-cells and weakly attractive for resting B-cells and memory (L-selectinlo) CD4 T-cells. May play a role in promoting encounters between recirculating T-cells and dendritic cells and in the migration of activated B-cells into the T-zone of secondary lymphoid tissues. Specifically binds to chemokine receptor CCR7.

Subcellular Location:
Secreted.

Tissue Specificity:
Highly expressed by dendritic cells in mesenteric and peripheral lymph nodes. Significant expression in spleen (T cell zone or periarteriolar lymphatic sheath) and Peyer patches. Low expression in thymus.

Similarity:
Belongs to the intercrine beta (chemokine CC) family.

Gene ID:
24047

CCL19,巨噬细胞炎性蛋白19抗体Database links:
Entrez Gene: 6363 Human
Entrez Gene: 100039053 Mouse
Entrez Gene: 24047 Mouse
Omim: 602227 Human
SwissProt: Q99731 Human
SwissProt: O70460 Mouse
Unigene: 50002 Human
Unigene: 426373 Mouse
Unigene: 441169 Mouse
Unigene: 459263 Mouse
Unigene: 6660 Mouse

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Tissue/cell: mouse intestine tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-CCL19 Polyclonal Antibody, Unconjugated(HZ-2454R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining mouse intestine tissue;4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-CCL19 Polyclonal Antibody, Unconjugated(HZ-2454R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(HZ-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei