CHRNA2(neuronal),烟碱型乙酰胆碱受体A2(神经型)抗体

产品编号HZ-2538R
英文名称CHRNA2(neuronal)
中文名称烟碱型乙酰胆碱受体A2(神经型)抗体
别 名Nicotinic Acetylcholine Receptor alpha 2; Nicotinic-Acetylcholine receptor; Chrna2; ACHR; CHRNA2; neuronal acetylcholine receptor subunit alpha-2 precursor; Acra-2; Acra2; BC011490; MGC18795; ACHA2_HUMAN; Cholinergic receptor nicotinic alpha 2; Neuronal acetylcholine receptor protein subunit alpha 2; Neuronal acetylcholine receptor subunit alpha-2.
说 明 书0.1ml 0.2ml
研究领域细胞生物 免疫学 神经生物学 通道蛋白 细胞膜受体
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Mouse, Rat, Dog, Rabbit,
CHRNA2(neuronal),烟碱型乙酰胆碱受体A2(神经型)抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量57kDa
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human CHRNA2
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
CHRNA2(neuronal),烟碱型乙酰胆碱受体A2(神经型)抗体PubMedPubMed
产品介绍background:
Nicotinic acetylcholine receptors (nAChRs) are ligand-gated ion channels formed by a pentameric arrangement of alpha and beta subunits to create distinct muscle and neuronal receptors. Neuronal receptors are found throughout the peripheral and central nervous system where they are involved in fast synaptic transmission. This gene encodes an alpha subunit that is widely expressed in the brain. The proposed structure for nAChR subunits is a conserved N-terminal extracellular domain followed by three conserved transmembrane domains, a variable cytoplasmic loop, a fourth conserved transmembrane domain, and a short C-terminal extracellular region. Mutations in this gene cause autosomal dominant nocturnal frontal lobe epilepsy type 4. Single nucleotide polymorphisms (SNPs) in this gene have been associated with nicotine dependence. [provided by RefSeq].

Function:
After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.

Subunit:
Neuronal AChR seems to be composed of two different types of subunits: alpha and non-alpha (beta). Alpha-2 subunit can be combined to beta-2 or beta-4 to give rise to functional receptors.

Subcellular Location:
Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.

CHRNA2(neuronal),烟碱型乙酰胆碱受体A2(神经型)抗体DISEASE:
Epilepsy, nocturnal frontal lobe, 4 (ENFL4) [MIM:610353]: An autosomal dominant focal epilepsy characterized by nocturnal seizures associated with fear sensation, tongue movements, and nocturnal wandering, closely resembling nightmares and sleep walking. Note=The disease is caused by mutations affecting the gene represented in this entry.

Similarity:
Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-2/CHRNA2 sub-subfamily.

Gene ID:
1135

Database links:
Entrez Gene: 1135 Human
Omim: 118502 Human
SwissProt: Q5IS52 Chimpanzee
SwissProt: Q15822 Human
Unigene: 57718 Human

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-CHRNA2(neuronal) Polyclonal Antibody, Unconjugated(HZ-2538R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining