Cbx8,染色质结合蛋白Cbx8抗体

产品编号HZ-3500R
英文名称Cbx8
中文名称染色质结合蛋白Cbx8抗体
别 名Chromobox protein homolog 8; HPC3; Pc3; Polycomb 3 homolog; RC1; Rectachrome 1; CBX8_HUMAN.
说 明 书0.1ml 0.2ml
研究领域肿瘤 免疫学 信号转导 干细胞 转录调节因子 结合蛋白
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep,
产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
Cbx8,染色质结合蛋白Cbx8抗体分 子 量43kDa
细胞定位细胞核
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human Cbx8
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Cbx8,染色质结合蛋白Cbx8抗体PubMedPubMed
产品介绍background:
Cbx8, also known as Pc3, is a member of the Polycomb gene family and is required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. Cbx8 contains a chromodomain and is able to modify chromatin and histones.

Function:
Component of a Polycomb group (PcG) multiproteinPRC1-like complex, a complex class required to maintain thetranscriptionally repressive state of many genes, including Hoxgenes, throughout development. PcG PRC1 complex acts via chromatinremodeling and modification of histones; it mediatesmonoubiquitination of histone H2A 'Lys-119', rendering chromatinheritably changed in its expressibility.

Subunit:
Component of a PRC1-like complex. Interacts with RING1RNF2, PCGF1, PCGF2, PCGF3, BMI1, PCGF5 AND PCGF6. Interacts withMLLT3 and histone H3. Interacts with PHC2 (By similarity).

Subcellular Location:
Nucleus.

Similarity:
Contains 1 chromo domain.

Gene ID:
57332

Cbx8,染色质结合蛋白Cbx8抗体Database links:
Entrez Gene: 506458 Cow
Entrez Gene: 57332 Human
Entrez Gene: 30951 Mouse
Entrez Gene: 303731 Rat
SwissProt: Q9HC52 Human
SwissProt: Q9QXV1 Mouse
Unigene: 387258 Human
Unigene: 99953 Mouse
Unigene: 43722 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-Cbx8 Polyclonal Antibody, Unconjugated(HZ-3500R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining