CKMT2,肌酸磷酸激酶2抗体

产品编号HZ-3526R
英文名称CKMT2
中文名称肌酸磷酸激酶2抗体
别 名CKMT 2; Basic-type mitochondrial creatine kinase; CKMT 2; CKMT2; CPK; Creatine kinase mitochondrial 2; Creatine kinase mitochondrial 2 (sarcomeric); Creatine kinase S-type; creatine kinase S-type, mitochondrial; Creatine kinase, sarcomeric mitochondrial; KCRS_HUMAN; Mib CK; Mib-CK; mitochondrial; OTTHUMP00000147542; S-MtCK; Sarcomeric mitochondrial creatine kinase; SMTCK.
说 明 书0.1ml 0.2ml
研究领域心血管 细胞生物 免疫学 转录调节因子 激酶和磷酸酶 线粒体
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit,
CKMT2,肌酸磷酸激酶2抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量46kDa
细胞定位细胞浆 细胞膜 线粒体
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human CKMT2
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
CKMT2,肌酸磷酸激酶2抗体PubMedPubMed
产品介绍background:
CKMT2 (creatine kinase, mitochondrial 2 (sarcomeric)) is a creatine kinase isoenzyme, (reversibly) catalyzing the production of ATP from ADP by converting Phosphocreatine to creatine. Phosphocreatine serves as an energy reservoir for the rapid generation of ATP.Mitochondrial creatine kinase exists as two isoenzymes, sarcomeric mitochondrial creatine kinase (CKMT2) and ubiquitous creatine kinase; these are encoded by separate genes. Mitochondrial creatine kinases occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Sarcomeric mitochondrial creatine kinase has 80% homology with the coding exons of ubiquitous mitochondrial creatine kinase. The CKMT2 gene contains sequences homologous to several motifs that are shared among some nuclear genes encoding mitochondrial proteins and thus may be essential for the coordinated activation of these genes during mitochondrial biogenesis. Three transcript variants encoding the same protein have been found for this gene.

Function:
Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.

Subunit:
Exists as an octamer composed of four CKMT2 homodimers.

Subcellular Location:
Mitochondrion inner membrane; Peripheral membrane protein; Intermembrane side.

Tissue Specificity:
Sarcomere-specific. Found only in heart and skeletal muscles.

Similarity:
Belongs to the ATP:guanido phosphotransferase family.
Contains 1 phosphagen kinase C-terminal domain.
Contains 1 phosphagen kinase N-terminal domain.

Gene ID:
1160

CKMT2,肌酸磷酸激酶2抗体Database links:
Entrez Gene: 538944 Cow
Entrez Gene: 1160 Human
Entrez Gene: 76722 Mouse
Entrez Gene: 688698 Rat
Omim: 123295 Human
SwissProt: Q3ZBP1 Cow
SwissProt: P17540 Human
SwissProt: Q6P8J7 Mouse
SwissProt: P09605 Rat
Unigene: 80691 Human
Unigene: 316438 Mouse
Unigene: 162549 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Tissue/cell: rat heart tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-CKMT2 Polyclonal Antibody, Unconjugated(HZ-3526R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining