CYBR1,细胞色素B还原酶1抗体

产品编号HZ-8297R
英文名称CYBR1
中文名称细胞色素B还原酶1抗体
别 名CYBR-1; CYBR 1; CYBR1_HUMAN; CYBRD 1; CYBRD1; Cytochrome b reductase 1; DCYTB; DKFZp564E227; Duodenal cytochrome b; Ferric chelate reductase 3; Ferric-chelate reductase 3; FLJ23462; FRRS3.
说 明 书0.1ml 0.2ml
研究领域细胞生物 免疫学 信号转导
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse,
CYBR1,细胞色素B还原酶1抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量31kDa
细胞定位细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human CYBR1/Cytochrome b reductase 1
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
CYBR1,细胞色素B还原酶1抗体PubMedPubMed
产品介绍background:
Ferric-chelate reductase that reduces Fe(3+) to Fe(2+). Present at the brush border of duodenal enterocytes where it probably reduces dietary Fe(3+) thereby facilitating its transport into the mucosal cells. Uses ascorbate as electron donor. May be involved in extracellular ascorbate recycling in erythrocyte membranes. May also act as a ferrireductase in airway epithelial cells.

Function:
Ferric-chelate reductase that reduces Fe(3+) to Fe(2+). Present at the brush border of duodenal enterocytes where it probably reduces dietary Fe(3+) thereby facilitating its transport into the mucosal cells. Uses ascorbate as electron donor. May be involved in extracellular ascorbate recycling in erythrocyte membranes. May also act as a ferrireductase in airway epithelial cells.

Subcellular Location:
Membrane; Multi-pass membrane protein.

Tissue Specificity:
Present in erythrocyte membranes (at protein level). Also expressed in respiratory epithelium.

Similarity:
Contains 1 cytochrome b561 domain.

CYBR1,细胞色素B还原酶1抗体Database links:
UniProtKB/Swiss-Prot: Q53TN4.1
Entrez Gene: 79901 Human
Entrez Gene: 73649 Mouse
Entrez Gene: 295669 Rat
Omim: 605745 Human
SwissProt: Q53TN4 Human
SwissProt: Q925G2 Mouse
SwissProt: Q5RKJ2 Rat
Unigene: 726027 Human
Unigene: 741213 Human
Unigene: 45435 Mouse
Unigene: 203500 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-CYBR1 Polyclonal Antibody, Unconjugated(HZ-8297R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining