LEU5,白血病相关蛋白5抗体

产品编号HZ-6395R
英文名称LEU5
中文名称白血病相关蛋白5抗体
别 名B cell chronic lymphocytic leukemia tumor suppressor Leu5; CAR; DLEU5; HGNC:9976; LEU5; Leukemia associated protein 5; Putative tumor suppressor RFP2; Ret finger protein 2; RING finger protein 77; RNF77; TRIM13; Tripartite motif protein 13; TRI13_HUMAN.
说 明 书0.1ml 0.2ml
研究领域肿瘤 细胞生物 染色质和核信号 细胞周期蛋白 转录调节因子 表观遗传学
抗体来源Rabbit
克隆类型Polyclonal
LEU5,白血病相关蛋白5抗体交叉反应 Human, Mouse, Rat, Dog, Pig, Horse, Rabbit, Sheep,
产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量47kDa
细胞定位细胞浆 细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human LEU5/RFP2
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
LEU5,白血病相关蛋白5抗体PubMedPubMed
产品介绍background:
This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This gene is located on chromosome 13 within the minimal deletion region for B-cell chronic lymphocytic leukemia. Multiple alternatively spliced transcript variants have been found for this gene.

Function:
E3 ubiquitin ligase involved in the retrotranslocation and turnover of membrane and secretory proteins from the ER through a set of processes named ER-associated degradation (ERAD). This process acts on misfolded proteins as well as in the regulated degradation of correctly folded proteins. Enhances ionizing radiation-induced p53/TP53 stability and apoptosis via ubiquitinating MDM2 and AKT1 and decreasing AKT1 kinase activity through MDM2 and AKT1 proteasomal degradation. Regulates ER stress-induced autophagy, and may act as a tumor suppressor.

Subunit:
Interacts (via C-terminal domain) with VCP. Interacts with AKT1; the interaction ubiquitinates AKT1 and leads to its proteasomal degradation. Interacts with MDM2; the interaction ubiquitinates AKT1 and leads to its proteasomal degradation. Interacts with p62/SQSTM1.

LEU5,白血病相关蛋白5抗体Subcellular Location:
Endoplasmic reticulum membrane; Single-pass membrane protein. Note=Concentrates and colocalizes with p62/SQSTM1 and ZFYVE1 at the perinuclear endoplasmic reticulum.

Post-translational modifications:
Auto-ubiquitinated; requires the RING-type zinc finger. Auto-polyubiquitination leads to proteasomal degradation.

Similarity:
Belongs to the TRIM/RBCC family.
Contains 1 B box-type zinc finger.
Contains 1 RING-type zinc finger.

Database links:
UniProtKB/Swiss-Prot: O60858.2

LEU5,白血病相关蛋白5抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.