Proteasome 20S alpha 3(Ser250),磷酸化蛋白酶体PSMα3抗体

产品编号HZ-9353R
英文名称Proteasome 20S alpha 3(Ser250)
中文名称磷酸化蛋白酶体PSMα3抗体
别 名Proteasome 20S alpha 3 (phospho S250); 20S Proteasome α3; HC8; Macropain subunit C8; Multicatalytic endopeptidase complex subunit C8; Proteasome (prosome macropain) subunit alpha type 3; Proteasome alpha 3 subunit; Proteasome component C8; Proteasome subunit alpha type 3; Proteasome subunit alpha type-3; Proteasome subunit C8; PSA3_HUMAN; PSC3; PSMA3.
说 明 书0.1ml
产品类型磷酸化抗体
研究领域细胞生物 细胞周期蛋白 细胞分化
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep,
Proteasome 20S alpha 3(Ser250),磷酸化蛋白酶体PSMα3抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量28kDa
细胞定位细胞核 细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated Synthesised phosphopeptide derived from human Proteasome 20S alpha 3 around the phosphorylation site of Ser250
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Proteasome 20S alpha 3(Ser250),磷酸化蛋白酶体PSMα3抗体PubMedPubMed
产品介绍background:
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Binds to the C-terminus of CDKN1A and thereby mediates its degradation. Negatively regulates the membrane trafficking of the cell-surface thromboxane A2 receptor (TBXA2R) isoform 2.

Function:
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Binds to the C-terminus of CDKN1A and thereby mediates its degradation. Negatively regulates the membrane trafficking of the cell-surface thromboxane A2 receptor (TBXA2R) isoform 2.

Subunit:
The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel. Interacts with AURKB. Interacts with CDKN1A. Interacts with HIV-1 TAT protein. Interacts with hepatitis C virus (HCV) F protein. Interacts with Epstein-Barr virus EBNA3 proteins. Interacts with MDM2 and RB1. Interacts with the C-terminus of TBXA2R isoform 2.

Subcellular Location:
Cytoplasm. Nucleus.

Proteasome 20S alpha 3(Ser250),磷酸化蛋白酶体PSMα3抗体Similarity:
Belongs to the peptidase T1A family.

Gene ID:
5684

Database links:
Entrez Gene: 5684 Human
Entrez Gene: 19167 Mouse
Entrez Gene: 29670 Rat
Entrez Gene: 408248 Rat
Omim: 176843 Human
SwissProt: P25788 Human
SwissProt: O70435 Mouse
SwissProt: P18422 Rat
Unigene: 558799 Human
Unigene: 296338 Mouse
Unigene: 3997 Rat

Proteasome 20S alpha 3(Ser250),磷酸化蛋白酶体PSMα3抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.