RUBISCO,核酮糖1,5二磷酸羧化酶抗体

产品编号HZ-6988R
英文名称RUBISCO
中文名称核酮糖1,5二磷酸羧化酶抗体
别 名rbcL; RBL_SPIOL; Ribulose bisphosphate carboxylase large chain; RuBisCO large subunit; ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit.
说 明 书0.1ml 0.2ml
研究领域细胞生物 免疫学 信号转导
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Arabidopsis Thaliana,
RUBISCO,核酮糖1,5二磷酸羧化酶抗体产品应用WB=1:500-1000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量52kDa
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from Arabidopsis thaliana RUBISCO
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
RUBISCO,核酮糖1,5二磷酸羧化酶抗体PubMedPubMed
产品介绍background:
RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate in the photorespiration process. Both reactions occur simultaneously and in competition at the same active site.
Sequence similarities: Belongs to the RuBisCO large chain family. Type I subfamily.
Post-translational modifications: The disulfide bond which can form between Cys-247 in the large chain dimeric partners within the hexadecamer appears to be associated with oxidative stress and protein turnover (By similarity). The disulfide bonds reported in 1RBO may be the result of oxidation during crystallization.

Function:
RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate in the photorespiration process. Both reactions occur simultaneously and in competition at the same active site.

Subunit:
Heterohexadecamer of 8 large chains and 8 small chains; disulfide-linked. The disulfide link is formed within the large subunit homodimers.

Subcellular Location:
Plastid, chloroplast.

RUBISCO,核酮糖1,5二磷酸羧化酶抗体Post-translational modifications:
The disulfide bond which can form between Cys-247 in the large chain dimeric partners within the hexadecamer appears to be associated with oxidative stress and protein turnover (By similarity). The disulfide bonds reported in 1RBO may be the result of oxidation during crystallization.

Similarity:
Belongs to the RuBisCO large chain family. Type I subfamily.

Database links:

UniProtKB/Swiss-Prot: P00875.1

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.