Wnt16,信号通路Wnt16抗体

产品编号HZ-9476R
英文名称Wnt16
中文名称信号通路Wnt16抗体
别 名Protein Wnt 16 precursor; Protein Wnt-16; Wingless type MMTV integration site family member 16; WNT16; WNT16_HUMAN.
说 明 书0.1ml 0.2ml
研究领域肿瘤 免疫学 信号转导 转录调节因子 糖蛋白
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Pig, Cow, Rabbit,
Wnt16,信号通路Wnt16抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量37kDa
细胞定位细胞外基质 分泌型蛋白
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human Wnt16
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Wnt16,信号通路Wnt16抗体PubMedPubMed
产品介绍background:
The Wnt genes encode a family of secreted extracellular signaling glycoproteins, which function in a variety of important developmental processes such as regulation of cell growth and differentiation. Wnt proteins also play roles in carcinogenesis. Wnt-14, rather than Wnt-15, is preferentially expressed in various types of human cancer. Wnt-15 is expressed in fetal and adult kidney and is most homologous to Wnt-14. Wnt-16, another member in the Wnt family, has two mRNA isoforms, Wnt-16a and Wnt-16b. These isoforms differ in the composition of their 5'UTR and first exon, which results in differential expression. Wnt-16a is expressed only in pancreas, whereas Wnt-16b is highly expressed in adult kidney, placenta, brain, heart and spleen, but not in bone marrow. However, Wnt-16 transcripts are present in bone marrow and cell lines derived from pre-B acute lymphoblastoid leukemia patients carrying the E2A-Pbx1 hybrid gene. Thus, Wnt-16 is a downstream target of E2A-Pbx1, and the Wnt-16-mediated autocrine growth mechanism may contribute to the development of t(1;19) pre-B acute lymphoblastoid leukemias.

Function:
Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters

Subcellular Location:
Secreted.extracellular space, extracellular matrix.

Tissue Specificity:
Isoform Wnt-16b is expressed in peripheral lymphoid organs such as spleen, appendix, and lymph nodes, in kidney but not in bone marrow. Isoform Wnt-16a is expressed at significant levels only in the pancreas.

Post-translational modifications:
Palmitoylation at Ser-227 is required for efficient binding to frizzled receptors. It is also required for subsequent palmitoylation at Cys-81. Palmitoylation is necessary for proper trafficking to cell surface

Wnt16,信号通路Wnt16抗体Similarity:
Belongs to the Wnt family.

Database links:
Entrez Gene: 51384 Human
Entrez Gene: 93735 Mouse
Entrez Gene: 500047 Rat
Omim: 606267 Human
SwissProt: Q9UBV4 Human
SwissProt: Q9QYS1 Mouse
Unigene: 272375 Human
Unigene: 137403 Mouse
Unigene: 209854 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-Wnt16 Polyclonal Antibody, Unconjugated(HZ-9476R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining