KLC1,驱动蛋白2抗体

产品编号HZ-11212R
英文名称KLC1
中文名称驱动蛋白2抗体
别 名Kinesin 2 60/70kDa; Kinesin light chain 1; Kinesin2; Kinesin-2; KLC 1; KLC; KLC1; KLC1_HUMAN; KNS 2; KNS 2A; KNS2; KNS2A; Medulloblastoma antigen MU MB 2.50; MGC15245.
说 明 书0.1ml 0.2ml
研究领域神经生物学 信号转导 细胞骨架 细胞外基质
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Cow, Horse, Rabbit, Zebrafish, Sheep,
KLC1,驱动蛋白2抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量65kDa
细胞定位细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human KLC1
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
KLC1,驱动蛋白2抗体PubMedPubMed
产品介绍background:
The kinesin family of motor proteins comprise at least two forms of conventional kinesin (kinesin-I). They are encoded by different genes and designated ubiquitous kinesin, which is expressed in all cells and tissues, and neuronal kinesin, which is expressed exclusively in neuronal cells. Conventional kinesin is a heterotetramer of two kinesin heavy chain subunits and two kinesin light chain subunits. While the kinesin heavy chain contains motor activity, evidence suggests that the kinesin light chain is involved in either modulation of kinesin heavy chain activity or in cargo binding. The motor protein kinesin is a heterotetramer composed of two heavy chains and two light chains. Kinesin motor activity is dependent on the presence of ATP and microtubules.

Function:
Kinesin is a microtubule-associated force-producing protein that may play a role in organelle transport. The light chain may function in coupling of cargo to the heavy chain or in the modulation of its ATPase activity.

Subunit:
Belongs to the kinesin light chain family. Contains 6 TPR repeats.

Subcellular Location:
Cytoplasm; cytoskeleton.

Tissue Specificity:
Found in a variety of tissues. Mostly abundant in brain and spine.

Post-translational modifications:
Isoform I is phosphorylated on Ser-600. Isoform J is phosphorylated on Ser-631.

Similarity:
Belongs to the kinesin light chain family.
Contains 6 TPR repeats.

Gene ID:
3831

KLC1,驱动蛋白2抗体Database links:
Entrez Gene: 3831 Human
Entrez Gene: 16593 Mouse
Entrez Gene: 171041 Rat
Omim: 600025 Human
SwissProt: Q07866 Human
SwissProt: O88447 Mouse
SwissProt: P37285 Rat
Unigene: 20107 Human
Unigene: 657678 Human
Unigene: 278357 Mouse
Unigene: 106230 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Protein: line1,Hela lysates;line2,293T lysate; Primary: rabbit Anti-KLC1 (HZ-11217R) at 1:300;  Secondary: HRP conjugated Goat-Anti-rabbit IgG(HZ-0295G-HRP) at 1: 5000;  Predicted band size:65 kD Observed band size:65 kD Tissue/cell: human laryngeal tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-KLC1 Polyclonal Antibody, Unconjugated(HZ-11212R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: human laryngeal tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-KLC1 Polyclonal Antibody, Unconjugated(HZ-11212R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining