SPAG17,精子相关抗原17抗体

产品编号HZ-9596R
英文名称SPAG17
中文名称精子相关抗原17抗体
别 名PF6; FLJ34497; Projection protein PF6 homolog; RP4 776P7.2; SPAG17; Sperm-associated antigen 17; SPG17_HUMAN.
说 明 书0.1ml 0.2ml
研究领域细胞生物 发育生物学 细胞骨架 细胞外基质
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat,
SPAG17,精子相关抗原17抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量252kDa
细胞定位细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human SPAG17
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
SPAG17,精子相关抗原17抗体PubMedPubMed
产品介绍background:
SPAG17 (sperm associated antigen 17), also known as PF6, is a 2,223 amino acid cytoplamic protein that colocalizes with SPAG6 to microtubles. Highly expressed in testis and in organs that contain cilia-bearing cells including brain, oviduct, lung, and uterus, SPAG17 may be important for the structural integrity of the central apparatus of the sperm axoneme. SPAG17 contains two LRR (leucine-rich) repeats and may also participate in flagellar motility and male fertility.

Function:
Seems to be important for the structural integrity of the central apparatus of the sperm axoneme.

Subunit:
Interacts (via the C-terminus) with SPAG6; the interaction probably occurs on polymerized microtubules (By similarity).

Subcellular Location:
Cytoplasm. Cytoplasm > cytoskeleton > flagellum axoneme. Detected in the cytoplasm of round spermatids and in condensing spermatids. Localized to the central pair of the sperm flagellar axoneme. Colocalizes with SPAG6 on microtubules.

Tissue Specificity:
Highly expressed in testis. Expressed in organs that contain cilia-bearing cells including brain, oviduct, lung, and uterus.

Database links:
Entrez Gene: 200162 Human
SwissProt: Q6Q759 Human
Unigene: 528821 Human

SPAG17,精子相关抗原17抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Protein: line1,mo brain lysates, 30ug;  Primary: Anti-SPAG17 (HZ-9596R) at 1:300;  Secondary: HRP conjugated Goat-Anti-Rabbit IgG(HZ-0295G) at 1: 5000;  ECL excitated the fluorescence;  Predicted band size : 57kD Observed band size : 57kD Tissue/cell: mouse brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-SPAG17 Polyclonal Antibody, Unconjugated(HZ-9596R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining