Slit1,多重表皮生长因子样蛋白4抗体

产品编号HZ-11487R
英文名称Slit1
中文名称多重表皮生长因子样蛋白4抗体
别 名MEGF 4; MEGF4; Multiple EGF like domains protein 4; Multiple epidermal growth factor like domains 4; Multiple epidermal growth factor like domains protein 4; SLIL 1; SLIL1; Slit 1; SLIT 3; Slit homolog 1; Slit homolog 1 protein; Slit-1; Slit1; SLIT3; KIAA0813; SLIT1_HUMAN.
说 明 书0.1ml 0.2ml
研究领域细胞生物 发育生物学 神经生物学
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Dog, Cow, Horse, Rabbit, Sheep,
Slit1,多重表皮生长因子样蛋白4抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量164kDa
细胞定位分泌型蛋白
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human Slit1/MEGF4 (455-500aa)
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Slit1,多重表皮生长因子样蛋白4抗体PubMedPubMed
产品介绍background:
Secreted leucine-rich repeat-containing proteins 1-3 (Slit1-3) are secreted glycoproteins that influence axonal guidance and mediate normal neural development by acting as high-affinity signaling ligands for the repulsive guidance receptor, Roundabout (Robo) (1, 2). Within the developing central nervous system (CNS) of different vertebrate systems, Slit proteins are expressed in equivalent regions, suggesting a conserved function among vertebrate homologs (3,4). Slit is expressed in the midline of the central nervous system in both vertebrates and invertebrates, where it functions as a regulatory factor of mesodermal cell movement during gastrulation (5). Slit2 is a short range inhibitory guidance cue for retinal ganglion cell (RGC) axons that may mediate spatial progression of RGCs (6,7).

Function:
Thought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions (By similarity). SLIT1 and SLIT2 together seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb.

Subunit:
Interacts with ROBO1 and GREM1

Subcellular Location:
Secreted.

Tissue Specificity:
Predominantly expressed in adult forebrain. Expressed in fetal brain, lung and kidney.

Similarity:
Contains 1 CTCK (C-terminal cystine knot-like) domain.
Contains 9 EGF-like domains.
Contains 1 laminin G-like domain.
Contains 20 LRR (leucine-rich) repeats.
Contains 4 LRRCT domains.
Contains 4 LRRNT domains.

Slit1,多重表皮生长因子样蛋白4抗体Database links:
Entrez Gene: 395293 Chicken
Entrez Gene: 6585 Human
Entrez Gene: 20562 Mouse
Entrez Gene: 65047 Rat
Entrez Gene: 380270 Xenopus laevis
Omim: 603742 Human
SwissProt: O75093 Human
SwissProt: Q80TR4 Mouse
SwissProt: O88279 Rat
Unigene: 632082 Human
Unigene: 40322 Mouse
Unigene: 30002 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Tissue/cell: Mouse brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-Slit1 Polyclonal Antibody, Unconjugated(HZ-11487R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: Mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-Slit1 Polyclonal Antibody, Unconjugated(HZ-11487R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining