FDFT1,法尼基二磷酸法尼基转移酶1/鲨烯合成酶抗体

产品编号HZ-11517R
英文名称FDFT1
中文名称法尼基二磷酸法尼基转移酶1/鲨烯合成酶抗体
别 名DGPT; ERG9; Farnesyl diphosphate farnesyltransferase 1; Farnesyl diphosphate farnesyltransferase; Farnesyl-diphosphate farnesyltransferase; FDFT_HUMAN; FDFT1; FPP:FPP farnesyltransferase; Presqualene di diphosphate synthase; SQS; Squalene synthase; Squalene synthetase; SS.
说 明 书0.1ml 0.2ml
研究领域肿瘤 细胞生物 神经生物学
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Dog,
FDFT1,法尼基二磷酸法尼基转移酶1/鲨烯合成酶抗体产品应用IHC-P=1:100-500 IHC-F=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量48kDa
细胞定位细胞浆 细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human FDFT1
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
FDFT1,法尼基二磷酸法尼基转移酶1/鲨烯合成酶抗体PubMedPubMed
产品介绍background:
Several proteins mediate the biosynthesis of cholesterol. The first specific step in the cholesterol biosynthetic pathway is the conversion of transfarnesyl-diphosphate to Squalene, which is catalyzed by the endoplasmic reticulum membrane-associated enzyme Squalene synthetase, also designated Squalene synthase and Farnesyl-diphosphate farnesyltransferase. Squalene synthetase is located at a branch point in the mevalonate pathway and is also involved in isoprenoid biosynthesis. Squalene epoxidase, also designated Squalene monooxygenase, is a multi-pass microsomal membrane-associated enzyme that catalyzes the first oxygenation step in sterol biosynthesis and most likely functions as one of the rate-limiting enzymes in this pathway. Squalene epoxidase may form a complex with Squalene synthetase.

Function:
Terpene metabolism; lanosterol biosynthesis; lanosterol from farnesyl diphosphate: step 1/3.

Subunit:
Monomer

Subcellular Location:
Endoplasmic reticulum membrane.

Similarity:
Belongs to the phytoene/squalene synthase family.

Gene ID:
2222

Database links:
Entrez Gene: 2222 Human
Entrez Gene: 14137 Mouse
Omim: 184420 Human
SwissProt: P37268 Human
SwissProt: P53798 Mouse
Unigene: 593928 Human
Unigene: 474432 Mouse

FDFT1,法尼基二磷酸法尼基转移酶1/鲨烯合成酶抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Farnesyl diphosphate farnesyltransferase; FDFT1) Polyclonal Antibody, Unconjugated (HZ-11517R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.