SIM1,转录因子蛋白SIM1抗体

产品编号HZ-11585R
英文名称SIM1
中文名称转录因子蛋白SIM1抗体
别 名bHLHe14; Class E basic helix-loop-helix protein 14; SIM1; SIM1_HUMAN; Single minded homolog 1; Single-minded homolog 1.
说 明 书0.1ml 0.2ml
研究领域细胞生物 神经生物学 信号转导 转录调节因子
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Zebrafish,
SIM1,转录因子蛋白SIM1抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量85kDa
细胞定位细胞核
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human SIM1 (2-80aa)
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
SIM1,转录因子蛋白SIM1抗体PubMedPubMed
产品介绍background:
The Per-Arnt-Sim (PAS) domain was identified as a 270 amino acid motif that mediates associations between various PAS family transcription factors. Several PAS domain family members have been identified including AhR, Arnt 1, and single-minded proteins (SIM1 and SIM2). The aromatic (aryl) hydrocarbon receptor, AhR, is a ligand dependent transcription factor that interacts with specific DNA sequences termed xenobiotic responsive elements (XREs) to activate several genes including CYP1A1, glutathione S-transferase Ya subunit and DT-diaphorase. The Ah receptor nuclear translocator protein 1 (Arnt 1) is required for ligand-dependent nuclear translocation of the Ah receptor and is also necessary for Ah receptor binding to the XRE element. Both SIM1 and SIM2 inhibit AhR/Arnt dimerization, thus inhibiting transcriptional activation. The SIM genes are thought to be involved in the directing and regionalization of tissues during development and the SIM2 gene, which is located on chromosome 21, is a candidate for the gene responsible for Down syndrome.

Function:
Transcriptional factor that may have pleiotropic effects during embryogenesis and in the adult.

Subunit:
Efficient DNA binding requires dimerization with another bHLH protein. Heterodimer of SIM1 and ARNT.

Subcellular Location:
Nucleus.

Similarity:
Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 PAC (PAS-associated C-terminal) domain.
Contains 2 PAS (PER-ARNT-SIM) domains.
Contains 1 Single-minded C-terminal domain.

Gene ID:
6492

SIM1,转录因子蛋白SIM1抗体Database links:
Entrez Gene: 6492 Human
Entrez Gene: 20464 Mouse
Entrez Gene: 309888 Rat
Omim: 603128 Human
SwissProt: P81133 Human
SwissProt: Q61045 Mouse
Unigene: 520293 Human
Unigene: 4774 Mouse

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.