Anti-Ras (G13D) Mouse Monoclonal Antibody武汉纽斯特生物技术有限公司

性能参数

产品名称: Anti-Ras (G13D) Mouse Monoclonal Antibody
英文名称:  Anti-Ras (G13D) Mouse Monoclonal Antibody
抗体货号: 26036
产品规格: 100ul
级    别: 生化级,
产品产地: 美国
品牌商标: NewEastbio
价    格: 4800元
抗原: A synthetic peptide from the internal region of Ras which includes the mutation of G13D,
抗原来源:  human origin.
抗体来源: 小鼠
适用物种: 脊椎动物
应用范围: ELISA, WB, IHC
更新时间: 2019/4/19 14:09:00
详细资料:  点击查看
浏览人数:485
诚信指数:78点
了解更多:进入公司展台
使用范围:仅限科研使用,不能应用于临床
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生物在线声明:以上所展示的信息由企业自行提供,内容的真实性、准确性和合法性由发布企业负责。生物在线对此不承担任何保证责任。

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武汉纽斯特生物技术有限公司
地址:
武汉市东湖新技术开发区光谷生物城B3-3栋2-3楼
邮编:
430074
电话:
027-87561033 手机 18627017297
传真:
027-87561022
联系人:
黄先生
所在区域:
湖北·武汉
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产品详细描述

 

Ras(G13D) [26038]
[100 µL]
 

Catalog Number:  26038
Gene Symbol:  RAS (G13D)
Description:   Anti-Ras (G13D) Mouse Monoclonal Antibody
Background:   Ras (G13D) is one type of Ras missense mutations at the codon 13. Ras protein is a membrane-associated GTPase that regulates cell proliferation, differentiation, and survival. Ras G13D mutation results in decreased GTPase activity and constitutive signaling. This mutation is found in many types of human cancers.
Immunogen:    A synthetic peptide from the internal region of Ras which includes the mutation of G13D, human origin.
Applications:   ELISA, WB, IF, IHC
Recommended Dilutions:   
ELISA: 1:100-1:3000
WB: 1:200-1:1000
IF: 1:50-1:100
IHC: 1:50-1:100
Concentration:   1 mg/ml
Host:   mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   IgG2b
Purity:   Purified from ascites
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  RasG13D antibody recognizes RasG13D proteins of vertebrates.
Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles
 
Western blot:  
Western blot analysis of recombinant Ras WT and G13D proteins with anti-Ras(G13D) monoclonal antibody. Equal amounts of purified Ras WT (lane 1, Cat # 10172) and G13D (lane 2, Cat # 10155) proteins were loaded and blotted with anti-Ras(G13D) mouse monoclonal antibody (Cat # 26038) (top panel). Input control (bottom panel) was blotted with rabbit polyclonal anti-Ras antibody (Cat # 21021).
Immunofluorescence:  
Immunofluorescence of cells expressing RAS proteins with anti-RAS (G13D) antibody. HEK293T cells were transfected with pCDNA3-GFP-Ras(G13D) plasmid,pCDNA3-GFP-Ras(WT)plasmid, pCDNA3-GFP-Ras(G13A) plasmid,pCDNA3-GFP-Ras(G13V) plasmid, pCDNA3-GFP-Ras(G13C)plasmid, pCDNA3-GFP-Ras(G12R)plasmid or pCDNA3-GFP-Ras(G13S)plasmid, then fixed and stained with anti-Ras(G13D) monoclonal antibody (Cat. # 26038).
 

 

Publications:
1.  DNA vaccine elicits an efficient antitumor response by targeting the mutant Kras in a transgenic mouse lung cancer model
    Gene Therapy 21, 888-896 (October 2014)
2.  Are all RAS mutations the same? Coexisting KRAS and NRAS mutations in a caecal adenocarcinoma and contiguous tubulovillous adenoma
    J Clin Pathol. 2015 Aug;68(8):657-60. doi: 10.1136

生物在线声明:以上所展示的信息由企业自行提供,内容的真实性、准确性和合法性由发布企业负责。生物在线对此不承担任何保证责任。

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