Product Highlight

• Configuration-specific monoclonal antibody that recognizes Gα ₁₃-GTP, but not Gα ₁₃-GDP
• The only available Gα ₁₃ activation assay kit directly measures the level of Gα ₁₃-GTP
• Providing reliable results with consistent reproducibility than other Gα ₁₃ activation assay kits based on certain downstream signaling pathways
• Providing shorter experimental time
• Could be used to monitor the activation of Gα ₁₃ in cells and in tissues by immunohistochemistry

Product Description

A structurally diverse repertoire of ligands, from photons to large peptides, activates GPCRs to elicit their physiological functions. Ligand-bound GPCRs, in turn, function as guanine nucleotide exchange factors catalyzing the exchange of GDP bound on the Gα subunit with GTP in the presence of Gβγ, causing the dissociation of the Gα subunit from the Gβγ dimer to form two functional units (α and Gβγ). Both Gα and Gβγ subunits signal to various cellular signaling pathways. Based on the sequence and functional homologies, G proteins are grouped into four families: G _s, G _i, G _q, and G ₁₂. As increasing numbers of effectors and interacting proteins for these G proteins have been identified, the physiological processes in which G proteins participate are multiplying.

Among the four subfamilies of G proteins, the function of G _12/13 subfamily is less well understood. In this family, there are two members, G ₁₂ and G ₁₃, that are expressed ubiquitously. Gα ₁₂ knockout mice appeared normal. Gα ₁₃ knockout mice displayed embryonic lethality (~E9.5). The Gα ₁₃-/- mouse embryos had defective vascular systems. G ₁₃ is also essential for receptor tyrosine kinase-induced migration of fibroblast and endothelial cells.

NewEast Biosciences Gα ₁₃ Activation Assay Kit provides a simple and fast tool to monitor the activation of Gα ₁₃. Each kit provides sufficient quantities to perform 20 assays.

 

Assay Principle

NewEast Biosciences Gα ₁₃ Activation Assay Kit bases on the configuration-specific anti-Gα ₁₃-GTP monoclonal antibody to measure the active Gα ₁₃-GTP levels, either from cell extracts or from in vitro GTPgamma;S loading Gα ₁₃ activation assays. Briefly, anti-active Gα ₁₃ mouse monoclonal antibody will be incubated with cell lysates containing Gα ₁₃-GTP. The bound active Gα ₁₃ will then be pulled down by protein A/G agarose. The precipitated active Gα ₁₃ will be detected by immunoblot analysis using anti-Gα ₁₃ rabbit polyclonal antibody.