Anti-RAS (G12S) Mouse Monoclonal Antibody-抗体-抗体-生物在线
Anti-RAS (G12S) Mouse Monoclonal Antibody

Anti-RAS (G12S) Mouse Monoclonal Antibody

商家询价

产品名称： Anti-RAS (G12S) Mouse Monoclonal Antibody

英文名称： Anti-RAS (G12S) Mouse Monoclonal Antibody

产品编号： 26186

产品价格： null

产品产地： 美国

品牌商标： NewEastbio

更新时间： null

使用范围： ELISA, WB, IHC

武汉纽斯特生物技术有限公司
  • 联系人 :
  • 地址 : 武汉市东湖新技术开发区光谷生物城B3-3栋2-3楼
  • 邮编 : 430074
  • 所在区域 : 湖北
  • 电话 : 186****7297
  • 传真 : 027-87561022
  • 邮箱 : neweast3@126.com
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Ras (G12S) [26186]
[100ul]		  

Catalog Number:  26186
Gene Symbol:  H-Ras; K-Ras; N-Ras
Description:   Anti-RAS (G12S) Mouse Monoclonal Antibody
Background:   Ras protein is a membrane-associated GTPase signaling protein that regulates proliferation, differentiation, and cell survival. Ras G12S mutation result in decreased GTPase activity and constitutive signaling, and is correlated with the development of pancreatic intraepithelial neoplasia and lung lesions.
Immunogen:   A synthetic peptide from the internal region of RAS which includes the mutation of G12S, human origin.
Applications:   ELISA, WB, IHC
Recommended Dilutions:   
ELISA: 1:1000-1:5000
WB: 1:500-1:1000
IHC: 1:50-1:100
Concentration:   1 mg/ml
Host:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   
Purity:   Purified from ascites
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Recognizes G12S mutant, but not wild-type RAS of vertebrates.
Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles
 
Western blot:  
Western blot analysis of recombinant RAS (G12S) and wildtype proteins. Purified His-tagged RAS (G12S) protein (lane 2) and corresponding wildtype protein (lane 1) were blotted with anti-RAS (G12S) monoclonal antibody (Cat. #26186).
Immunofluorescence:  
Immunofluorescence of cells expressing RAS proteins with anti-RAS (G12S) antibody. HEK293T cells were transfected with pCDNA3-GFP-Ras(G12S) plasmid, pCDNA3-GFP-Ras(WT)plasmid,pCDNA3-GFP-Ras(G12S) plasmid, pCDNA3-GFP-Ras(G12V)plasmid,pCDNA3-GFP-Ras(G12C)plasmid, pCDNA3-GFP-Ras(G12R)plasmid or pCDNA3-GFP-Ras(G12A)plasmid, then fixed and stained with anti-RAS (G12S) monoclonal antibody (Cat. #26186).

 

Publications:
1.  DNA vaccine elicits an efficient antitumor response by targeting the mutant Kras in a transgenic mouse lung cancer model
    Gene Therapy 21, 888-896 (October 2014)
2.  Are all RAS mutations the same? Coexisting KRAS and NRAS mutations in a caecal adenocarcinoma and contiguous tubulovillous adenoma
    J Clin Pathol. 2015 Aug;68(8):657-60. doi: 10.1136