Product Highlight

• Configuration-specific monoclonal antibody that recognizes Rab3-GTP, but not Rab3-GDP
• The only available Rab3 activation assay kit directly measures the level of Rab3-GTP
• Providing shorter experimental time
• Could be used to monitor the activation of Rab3 in cells and in tissues by immunohistochemistry

Product Description

Rab3 belongs to the Rab family of small GTPases. Rab3 is located in the late endosome, lysosome, cytoplasmic vesicle, phagosome and melanosome. It is involved in the late endocytic transport and contributes to the maturation of phagosomes.

Currently there is no direct assay to measure the activation of Rab3 GTPases.

NewEast Biosciences Rab3 Activation Assay Kit is based on the configuration-specific monoclonal antibody that specifically recognizes Rab3-GTP, but not Rab3-GDP. Given the high affinity of monoclonal antibodies to their antigens, the activation assay could be performed in a short time. This assay provides the reliable results with consistent reproducibility.

These anti- Rab3-GTP monoclonal antibodies can also be used to monitor the activation of Rab3 in cells and in tissues by immunohistochemistry.

NewEast Biosciences Rab3 Activation Assay Kit provides a simple and fast method to monitor the activation of Rab3. Each kit provides sufficient quantities to perform 20 assays.

 

Assay Principle

NewEast Biosciences Rab3 Activation Assay Kit bases on the configuration-specific anti-Rab3-GTP monoclonal antibody to measure the active Rab3-GTP levels, either from cell extracts or from in vitro GTPγS loading Rab3 activation assays. Briefly, anti-active Rab3 mouse monoclonal antibody will be incubated with cell lysates containing Rab3-GTP. The bound active Rab3 will then be pulled down by protein A/G agarose. The precipitated active Rab3 will be detected by immunoblot analysis using anti- Rab3 rabbit polyclonal antibody.

Publications:

1.  VASP promotes TGF-β activation of hepatic stellate cells by regulating Rab11 dependent plasma membrane targeting of TGF-β receptors     Hepatology Volume 61, Issue 1, pages 361–374, January 2015

2.  Rab5 Activity Regulates GLUT4 Sorting into Insulin-Responsive and Non-Insulin-Responsive Endosomal Compartments: A Potential Mechanism for Development of Insulin Resistance     Endocrinology. 2014 Sep;155(9):3315-28