Anti-SMAD4 (R361S) Mouse Monoclonal Antibody-抗体-抗体-生物在线
Anti-SMAD4 (R361S) Mouse Monoclonal Antibody

Anti-SMAD4 (R361S) Mouse Monoclonal Antibody

商家询价

产品名称： Anti-SMAD4 (R361S) Mouse Monoclonal Antibody

英文名称： Anti-SMAD4 (R361S) Mouse Monoclonal Antibody

产品编号： 26423

产品价格： null

产品产地： 美国

品牌商标： NewEastbio

更新时间： null

使用范围： ELISA, WB, IHC

武汉纽斯特生物技术有限公司
  • 联系人 :
  • 地址 : 武汉市东湖新技术开发区光谷生物城B3-3栋2-3楼
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  • 所在区域 : 湖北
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  • 邮箱 : neweast3@126.com
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SMAD4 (R361S) [26423]
[100ul]		  

Catalog Number:  26423
Gene Symbol:  SMAD4, DPC4, JIP, MADH4, MYHRS
Description:   Anti-SMAD4 (R361S) Mouse Monoclonal Antibody
Background:   SMAD4 family member 4, also known as SMAD4, is a protein that in human is encoded by the SMAD4 gene. It is often found mutated in many cancers. It acts as a tumor suppressor that functions in the regulation of the TGF-? signal transduction pathway, which negatively regulates growth of epithelial cells and the extracellular matrix (ECM). SMAD4 alterations have been found in multiploid colorectal cancer and pancreatic carcinoma. It is found inactivated in at least 50% of pancreatic cancers. It is also found mutated in the autosomal dominantdisease juvenile polyposis syndrome (JPS).
Immunogen:   A synthetic peptide from the internal region of SMAD4 which includes the mutation of R361S, human origin.
Applications:   ELISA, WB, IHC
Recommended Dilutions:   
ELISA: 1:1000-1:2000
WB: 1:500-1:1000
IHC: 1:50-1:100
Concentration:   1 mg/ml
Host:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   
Purity:   Purified from ascites
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Recognizes R361S mutant, but not wild-type SMAD4 of vertebrates
Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles
 
Western blot:  
Western blot analysis of recombinant SMAD4 (R361S) and wildtype proteins. Purified His-tagged SMAD4 (R361S) protein (lane1) and corresponding wild-type protein (lane2) were blotted with anti-SMAD4 (R361S) monoclonal antibody (Cat. #26423).
Immunofluorescence:  
Immunofluorescence of cells expressing SMAD4 proteins with anti-SMAD4 (R361S) antibody. HEK293T cells were transfected with pCDNA3-GFP-SMAD4 (R361S) plasmid pCDNA3-GFP-SMAD4 (WT) plasmid, pCDNA3-GFP-SMAD4 (R361H) plasmid or pCDNA3-GFP-SMAD4 (R361C) plasmid, then fixed and stained with anti-SMAD4 (R361S) monoclonal antibody (Cat. #26423).