Ran信号传导分子激活检测试剂盒-试剂盒-抗体-生物在线
Ran信号传导分子激活检测试剂盒

Ran信号传导分子激活检测试剂盒

商家询价

产品名称: Ran信号传导分子激活检测试剂盒

英文名称: Ran Activation Assay Kit

产品编号: 81701

产品价格: null

产品产地: 美国

品牌商标: NewEastBio

更新时间: null

使用范围: IPWB

武汉纽斯特生物技术有限公司
  • 联系人 :
  • 地址 : 武汉市东湖新技术开发区光谷生物城B3-3栋2-3楼
  • 邮编 : 430074
  • 所在区域 : 湖北
  • 电话 : 186****7297
  • 传真 : 027-87561022
  • 邮箱 : neweast3@126.com

 

Ran Activation Assay Kit [81701]
[20 Assays]
 

Product Highlight

  • Configuration-specific monoclonal antibody that recognizes Ran-GTP, but not Ran-GDP
  • The only available Ran activation assay kit directly measures the level of Ran-GTP
  • Providing reliable results with consistent reproducibility than other Ran activation assay kits based on certain downstream signaling pathways
  • Providing shorter experimental time
  • Could be used to monitor the activation of Ran in cells and in tissues by immunohistochemistry

Product Description

    Small GTPases are a super-family of cellular signaling regulators. Ran (Ras-related nuclear protein) is a member of the Ras-superfamily GTPases. Ran is invovled in the transport of proteins across the nuclear envelope, as well as in microtubule organization during mitosis.

    Currently the activation of Ran proteins is assayed with the binding of GTP-bound Ran to the Ran-binding domain (RBD) of Raf protein kinase. This method is based on the observation that the active, GTP-bound Ran could bind to the RBD of Raf. However, the reproducibility of this method is poor. This is partially due to the relatively quick hydrolysis of GTP to GDP during the assay procedure, and the low binding affinity of RBD to Ran-GTP.

    Currently there is no direct assay to measure the activation of Ran GTPases.

    NewEast Biosciences Ran Activation Assay Kit is based on the configuration-specific monoclonal antibody that specifically recognizes Ran-GTP, but not Ran-GDP. Given the high affinity of monoclonal antibodies to their antigens, the activation assay could be performed in a short time. This assay provides the reliable results with consistent reproducibility.

    These anti-Ran-GTP monoclonal antibodies can also be used to monitor the activation of Ran in cells and in tissues by immunohistochemistry.

    NewEast Biosciences Ran Activation Assay Kit provides a simple and fast method to monitor the activation of Ran. Each kit provides sufficient quantities to perform 20 assays.

     

Assay Principle

    NewEast Biosciences Ran Activation Assay Kit bases on the configuration-specific anti-Ran-GTP monoclonal antibody to measure the active Ran-GTP levels, either from cell extracts or from in vitro GTPΓS loading Ran activation assays. Briefly, anti-active Ran mouse monoclonal antibody will be incubated with cell lysates containing Ran-GTP. The bound active Ran will then be pulled down by protein A/G agarose. The precipitated active Ran will be detected by immunoblot analysis using anti-Ran rabbit polyclonal antibody.

    Publications:
    1.  NMDAR signaling facilitates the IPO5-mediated nuclear import of CPEB3
        Nucleic Acids Res. 2012 Sep 1;40(17):8484-98