Ras信号传导分子激活检测试剂盒-试剂盒-抗体-生物在线
Ras信号传导分子激活检测试剂盒

Ras信号传导分子激活检测试剂盒

商家询价

产品名称: Ras信号传导分子激活检测试剂盒

英文名称: Ras Activation Assay Kit

产品编号: 81101

产品价格: null

产品产地: 美国

品牌商标: NewEastBio

更新时间: null

使用范围: IPWB

武汉纽斯特生物技术有限公司
  • 联系人 :
  • 地址 : 武汉市东湖新技术开发区光谷生物城B3-3栋2-3楼
  • 邮编 : 430074
  • 所在区域 : 湖北
  • 电话 : 186****7297
  • 传真 : 027-87561022
  • 邮箱 : neweast3@126.com

 

Ras Activation Assay Kit [81101]
[20 Assays]
 

Product Highlight

  • Configuration-specific monoclonal antibody that recognizes Ras-GTP, but not Ras-GDP
  • The only available Ras activation assay kit directly measures the level of Ras-GTP
  • Providing reliable results with consistent reproducibility than other Ras activation assay kits based on certain downstream signaling pathways
  • Providing shorter experimental time
  • Could be used to monitor the activation of Ras in cells and in tissues by immunohistochemistry

Product Description

    Small GTPases are a super-family of cellular signaling regulators. Ras belongs to the Ras sub-family of GTPases that regulate cell growth, cell motility, and gene transcription. GTP binding increases the activity of Ras, and the hydrolysis of GTP to GDP renders it inactive.

    Currently the activation of Ras proteins is assayed with the binding of GTP-bound Ras to the Ras-binding domain (RBD) of Raf protein kinase. This method is based on the observation that the active, GTP-bound Ras could bind to the RBD of Raf. However, the reproducibility of this method is poor. This is partially due to the relatively quick hydrolysis of GTP to GDP during the assay procedure, and the low binding affinity of RBD to Ras-GTP.

    NewEast Biosciences Ras Activation Assay Kit is based on the configuration-specific monoclonal antibody that specifically recognizes Ras-GTP, but not Ras-GDP. Given the high affinity of monoclonal antibodies to their antigens, the activation assay could be performed in a much shorter time. This assay provides the reliable results with consistent reproducibility.

    These anti-Ras-GTP monoclonal antibody can also be used to monitor the activation of Ras in cells and in tissues by immunohistochemistry.

    NewEast Biosciences Ras Activation Assay Kit provides a simple and fast method to monitor the activation of Ras. Each kit provides sufficient quantities to perform 20 assays.

     

Assay Principle

    NewEast Biosciences Ras Activation Assay Kit bases on the configuration-specific anti-Ras-GTP monoclonal antibody to measure the active Ras-GTP levels, either from cell extracts or from in vitro GTPγS loading Ras activation assays. Briefly, anti-active Ras mouse monoclonal antibody will be incubated with cell lysates containing Ras-GTP. The bound active Ras will then be pulled down by protein A/G agarose. The precipitated active Ras will be detected by immunoblot analysis using anti-Ras mouse monoclonal antibody