Anti- EYA2 Mouse Monoclonal Antibody-抗体-抗体-生物在线
Anti- EYA2 Mouse Monoclonal Antibody

Anti- EYA2 Mouse Monoclonal Antibody

商家询价

产品名称： Anti- EYA2 Mouse Monoclonal Antibody

英文名称： Anti- EYA2 Mouse Monoclonal Antibody

产品编号： 26096

产品价格： null

产品产地： 美国

品牌商标： NewEastbio

更新时间： null

使用范围： ELISA, WB, IHC

武汉纽斯特生物技术有限公司
  • 联系人 :
  • 地址 : 武汉市东湖新技术开发区光谷生物城B3-3栋2-3楼
  • 邮编 : 430074
  • 所在区域 : 湖北
  • 电话 : 186****7297
  • 传真 : 027-87561022
  • 邮箱 : neweast3@126.com
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EYA2 [26096]
[100 µL]		  

Catalog Number:  26096
Gene Symbol:  EYA2
Description:   Anti- EYA2 Mouse Monoclonal Antibody
Background:   EYA2 may be involved in development of the eye. It promotes efficient DNA repair by dephosphorylating H2AX, promoting the recruitment of DNA repair complexes containing MDC1. Its function as histone phosphatase probably explains its role in transcription regulation during organogenesis. EYA2 coactivates SIX1 and seems to coactivate SIX2, SIX4 and SIX5. It seems to be involved in myogenesis together with SIX1 and DACH2.
Immunogen:   Human EYA2 protein
Applications:   ELISA, WB, IHC
Recommended Dilutions:   
ELISA: 1:1000-1:5000
WB: 1:100-1:1000
IHC: 1:50-1:100
Concentration:   0.8 mg/ml
Host:   mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   
Purity:   Purified from ascites
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg 2+ and Ca 2+ ), pH 7.4, 150 mM NaCl, 50% glycerol Species
Species Reactivity:  Anti-EYA2 antibody recognizes EYA2 of vertebrates.
Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles.
 
Western blot:  
Western blot analysis of recombinant full length EYA2 protein. Purified His-tagged EYA2 protein(Cat. #10195) was blotted with anti-EYA2 monoclonal antibody (Cat # 26096).
Immunohistochemistry:  

 

Immunohistochemical analysis of paraffin-embedded thyroid cancer tissue with anti-EYA2 monoclonal antibody (Cat. #26096). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:50) overnight at 4°C. A HRP-conjugated Goat anti-mouse IgG (dilution 1:50) was used as secondary antibody.