anti-GRB7 Mouse Monoclonal Antibody-抗体-抗体-生物在线
anti-GRB7 Mouse Monoclonal Antibody

anti-GRB7 Mouse Monoclonal Antibody

商家询价

产品名称： anti-GRB7 Mouse Monoclonal Antibody

英文名称： anti-GRB7 Mouse Monoclonal Antibody

产品编号： 26133

产品价格： null

产品产地： 美国

品牌商标： NewEastbio

更新时间： null

使用范围： ELISA, WB, IHC

武汉纽斯特生物技术有限公司
  • 联系人 :
  • 地址 : 武汉市东湖新技术开发区光谷生物城B3-3栋2-3楼
  • 邮编 : 430074
  • 所在区域 : 湖北
  • 电话 : 186****7297
  • 传真 : 027-87561022
  • 邮箱 : neweast3@126.com
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GRB7 [26133]
[100 µL]		  

Catalog Number:  26133
Gene Symbol:  GRB7
Description:   anti-GRB7 Mouse Monoclonal Antibody
Background:   Grb7 is an adaptor protein that interacts with epidermal growth factor receptor (EGFR) and ephrin receptors. It plays a role in the integrin signaling pathway and cell migration by binding with focal adhesion kinase (FAK). Grb7 gene is commonly co-amplified (present in excess copies) in breast cancers. Grb7 is over-expressed in testicular germ cell tumors, esophageal cancers, and gastric cancers. There are clues that it involved in cell migration and metastatic progression of human esophageal carcinomas.
Immunogen:   Purified GRB7 protein, human origin.
Applications:   ELISA, WB, IHC
Recommended Dilutions:   
ELISA: 1:1000-1:5000
WB: 1:100-1:1000
IHC: 1:50-1:200
Concentration:    0.5 mg/ml
Host:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   
Purity:   Purified from ascites
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Recognizes GRB7 of vertebrates.
Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles
 
Western blot:  
Western blot analysis of recombinant GRB7 protein. Purified His-tagged GRB7 protein was blotted with anti-GRB7 mouse monoclonal antibody (Cat. #26133).
Immunohistochemistry:  

 

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with anti-GRB7 monoclonal antibody (Cat. #26133). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:50 dilution) overnight at 4°C. A HRP-conjugated Goat anti-mouse IgG (1:50 dilution) was used as secondary antibody.