| Catalog Number: 21271 |
| Gene Symbol: B-RAF, B-RAF1, RAFB1 |
| Description: Anti-B-Raf Rabbit Polyclonal Antibody |
| Background: B-Raf is a member of the Raf family of Ser/Thr protein kinases. It functions downstream of Ras to regulate the MAP kinase signaling pathway. Mutations in the B-RAF gene cause diseases. Inherited mutations in B-RAF cause cardiofaciocutaneous syndrome. Acquired mutations in B-RAF have been found in cancers. |
| Immunogen: Purified B-Raf protein, human origin. |
| Applications: ELISA, WB, IHC |
Recommended Dilutions:
ELISA: 1:1000-1:5000
WB: 1:100-1:1000
IHC: 1:50-1:200 |
| Concentration: 0.1mg/ml |
| Host: Rabbit |
| Format: Liquid |
| Clonality: Polyclonal |
| Isotype: |
| Purity: Purified from serum |
| Storage buffer: |
| Preservative: no |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Recognizes B-Raf of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles |
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| Immunohistochemistry: |
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Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue with anti- B-Raf polyclonal antibody (Cat. #21271). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:50 dilution) overnight at 4°C. A HRP-conjugated Goat anti-mouse IgG (1:50 dilution) was used as secondary antibody |
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| Publications: |
1. Immunohistochemistry with a mutation-specific monoclonal antibody as a screening tool for the BRAFV600E mutational status in primary cutaneous malignant melanoma
Mod Pathol. 2013 Mar;26(3):414-20. |
2. Immunohistochemistry as a quick screening method for clinical detection of BRAF(V600E) mutation in melanoma patients
Tumor Biology. June 2014, Volume 35, Issue 6, pp 5727-5733 |
3. Prospective immunohistochemical analysis of BRAF V600E mutation in melanoma
human pathology. February 2015Volume 46, Issue 2, Pages 169–175 |
4. Comparison of 2 monoclonal antibodies for immunohistochemical detection of BRAF V600E mutation in malignant melanoma, pulmonary carcinoma, gastrointestinal carcinoma, thyroid carcinoma, and gliomas
human pathology. November 2013Volume 44, Issue 11, Pages 2563–2570 |
5. BRAF V600E Mutation-Specific Antibody: A Review
Seminars in Diagnostic Pathology |
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