Anti-BRaf (V600R) Mouse Monoclonal Antibody-抗体-抗体-生物在线
武汉纽斯特生物技术有限公司
Anti-BRaf (V600R) Mouse Monoclonal Antibody

Anti-BRaf (V600R) Mouse Monoclonal Antibody

商家询价

产品名称： Anti-BRaf (V600R) Mouse Monoclonal Antibody

英文名称： Anti-B-Raf (V600R) Mouse Monoclonal Antibody

产品编号： 26182

产品价格： null

产品产地： 美国

品牌商标： NewEastBio

更新时间： null

使用范围： ELISA、WB、 IHC

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B-Raf (V600R) [26182]
[100ul]		  

Catalog Number:  26182
Gene Symbol:  BRAF, BRAF1, RAFB1
Description:   Anti-B-Raf (V600R) Mouse Monoclonal Antibody
Background:   B-Raf is a member of the Raf family of Ser/Thr protein kinases. It functions downstream of Ras to regulate the MAP kinase signaling pathway. Mutations in the BRAF gene cause diseases. Inherited mutations in BRAF cause cardiofaciocutaneous syndrome. Acquired mutations in BRAF have been found in cancers.
Immunogen:   A synthetic peptide from the internal region of B-Raf (V600R), human origin.
Applications:    ELISA, WB, IHC
Recommended Dilutions:   
ELISA 1:1000-1:5000 
WB 1:500-1:1500 
IHC 1:100-1:200
Concentration:   0.9 mg/ml
Host:   mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   Unknown
Purity:   Purified from ascites
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Recognizes B-Raf V600R, but not wild-type B-Raf proteins.
Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles
 
Western blot:  
Western blot analysis of recombinant B-raf proteins. Purified His-tagged B-Raf (V600R) protein ( lane 2) and corresponding wild type protein (lane 1) were blotted with anti-B-Raf (V600R) monoclonal antibody (Cat. # 26182).

Publications:

1.  Immunohistochemistry with a mutation-specific monoclonal antibody as a screening tool for the BRAFV600E mutational status in primary cutaneous malignant melanoma
    Mod Pathol. 2013 Mar;26(3):414-20.

2.  Immunohistochemistry as a quick screening method for clinical detection of BRAF(V600E) mutation in melanoma patients
    Tumor Biology. June 2014, Volume 35, Issue 6, pp 5727-5733

3.  Prospective immunohistochemical analysis of BRAF V600E mutation in melanoma
    human pathology. February 2015Volume 46, Issue 2, Pages 169–175

4.  Comparison of 2 monoclonal antibodies for immunohistochemical detection of BRAF V600E mutation in malignant melanoma, pulmonary carcinoma, gastrointestinal carcinoma, thyroid carcinoma, and gliomas
    human pathology. November 2013Volume 44, Issue 11, Pages 2563–2570

5.  BRAF V600E Mutation-Specific Antibody: A Review
    Seminars in Diagnostic Pathology