Anti-NFE2L2 (E79Q) Mouse Monoclonal Antibody-抗体-抗体-生物在线

Anti-NFE2L2 (E79Q) Mouse Monoclonal Antibody

商家询价

产品名称： Anti-NFE2L2 (E79Q) Mouse Monoclonal Antibody

英文名称： Anti-NFE2L2 (E79Q) Mouse Monoclonal Antibody

产品编号： 26336

产品价格： null

产品产地：美国

品牌商标： NewEastBio

更新时间： null

使用范围： ELISA、WB、 IHC

武汉纽斯特生物技术有限公司

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产品详情

NFE2L2 (E79Q) [26336]
[100ul]

Catalog Number: 26336

Gene Symbol: NFE2L2, NRF2

Description: Anti-NFE2L2 (E79Q) Mouse Monoclonal Antibody

Background: NFE2L2 protein is encoded by NFE2L2 gene. It is a basic leucine zipper protein that regulates the expression of antioxidant proteins that protect against oxidative damage triggered by injury and inflammation. Several drugs that stimulate the NFE2L2 pathway are being studied for treatment of diseases that are caused by oxidative stress.

Immunogen: A synthetic peptide from the internal region of NFE2L2 which includes the mutation of E79Q, human origin.

Applications: ELISA, WB, IHC

Recommended Dilutions:
ELISA: 1:1000-1:2000
WB: 1:500-1:1000
IHC: 1:50-1:100

Concentration: 1 mg/ml

Host: Mouse

Format: Liquid

Clonality: Monoclonal

Isotype:

Purity: Purified from ascites

Storage buffer:

Preservative: no

Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Recognizes E79Q mutant, but not wild-type NFE2L2 of vertebrates.

Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles

Western blot:

Western blot analysis of recombinant NFE2L2 (E79Q) and wildtype proteins. Purified His-tagged NFE2L2 (E79Q) protein (lane2) and corresponding wild-type protein (lane1) were blotted with anti-NFE2L2 (E79Q) monoclonal antibody (Cat. #26336).

Immunofluorescence:

Immunofluorescence of cells expressing NFE2L2 proteins with anti-NFE2L2 (E79Q) antibody. HEK293T cells were transfected with pCDNA3-GFP-NFE2L2 (WT) plasmid (left column) or pCDNA3-GFP-NFE2L2 (E79Q) plasmid (right column), then fixed and stained with anti-NFE2L2 (E79Q) monoclonal antibody (Cat. #26336).