Anti-Arf1 Rabbit Polyclonal Antibody-抗体-抗体-生物在线
Anti-Arf1 Rabbit Polyclonal Antibody

Anti-Arf1 Rabbit Polyclonal Antibody

商家询价

产品名称： Anti-Arf1 Rabbit Polyclonal Antibody

英文名称： Anti-Arf1 Rabbit Polyclonal Antibody

产品编号： 21283

产品价格： null

产品产地： 美国

品牌商标： NewEastBio

更新时间： null

使用范围： ELISA、WB、 IHC

武汉纽斯特生物技术有限公司
  • 联系人 :
  • 地址 : 武汉市东湖新技术开发区光谷生物城B3-3栋2-3楼
  • 邮编 : 430074
  • 所在区域 : 湖北
  • 电话 : 186****7297
  • 传真 : 027-87561022
  • 邮箱 : neweast3@126.com
进入展台

 

Arf1 [21283]
[100 µL]		  

Catalog Number:  21283
Gene Symbol:  ARF1
Description:   Anti-Arf1 Rabbit Polyclonal Antibody
Background:   ARF1 (ADP-ribosylation factor 1) is a member of the ARF super-family. ARF genes encode small GTPases that increase the ADP-ribosyltransferase activity of cholera toxin and are critical for vesicular trafficking as activators of phospholipase D. ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport.
Immunogen:   Purified Arf1 protein, human origin.
Applications:   ELISA, WB, IHC
Recommended Dilutions:   
ELISA: 1:1000-1:5000 
WB: 1:100-1:1000 
IHC: 1:50-1:200
Concentration:   0.2mg/ml
Host:   Rabbit
Format:   Liquid
Clonality:   Polyclonal
Isotype:   
Purity:   Purified from serum
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Recognizes Arf1, Arf2 and Arf3 of vertebrates.
Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles
 
Western blot:  
Western blot analysis of recombinant Arf1 protein. Purified His-tagged Arf1 protein was blotted with anti-Arf1 rabbit polyclonal antibody (Cat. #21283).
Immunohistochemistry:  
Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue with anti-Arf1 polyclonal antibody (Cat. #21283). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:50 dilution) overnight at 4°C. A HRP-conjugated Goat anti-mouse IgG (1:50 dilution) was used as secondary antibody.