【描述】

Monoclonal antibodies specific to six histidine tags can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify, detect, and purify polyhistidine fusion proteins in bacteria, insect cells, and mammalian cells. Anti His-tag mouse mAb recognizes His-tag placed at N-terminus, C-terminus, and internal regions of fusion proteins.

【亚型】Mouse IgG1

【目标蛋白】A synthetic peptide HHHHHH coupled to KLH.

【成份】1mg/ml in PBS, pH 7.4, containing 0.1% proclin and 50% glycerol

【贮存方法】Store at-20°C

【推荐稀释度】

Western Blot: 1:1000 ~1:10000
IP: 1:100 ~ 1:500
IF: 1:200 ~ 1:1000
Optimal dilutions should be determined by the end user.

Fig: Western blot analysis of His-tag fusion protein with anti His-tag mouse mAb (5B8) diluted at 1: 2000(lane1) and 1: 5000(lane2).

Anti His-Tag Mouse mAb抗体说明书