Rabbit Anti-Phospho-PLCG 2 (Tyr753) antibody
|英文名称||Phospho-PLCG 2 (Tyr753)|
|别 名||PLCG 2 (phospho Y753); p-PLCG 2 (phospho Y753); PLCG2(phospho Y753); PLCG 2(Phospho Tyr753); PLC 2; PLC gamma 2; PLC IV; PLCG2; 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 2; EC 220.127.116.11; Phosphoinositide phospholipase C; Phospholipase C gamma 2; Phospholipase C, gamma 2 (phosphatidylinositol specific).|
|研究领域||肿瘤 免疫学 信号转导 激酶和***酸酶|
|交叉反应||Human, Mouse, Rat, Pig, Cow, Horse, Rabbit,|
|产品应用||WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
|分 子 量||139kDa|
|性 状||Lyophilized or Liquid|
|免 疫 原||KLH conjugated Synthesised phosphopeptide derived from human PLC gamma 2 around the phosphorylation site of Tyr753:SL(p-Y)DV|
|纯化方法||affinity purified by Protein A|
|储 存 液||0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.|
|保存条件||Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.|
Enzymes of the phospholipase C family catalyze the hydrolysis of phospholipids to yield diacylglycerols and water soluble phosphorylated derivatives of the lipid head groups. A number of these enzymes have specificity for phosphoinositides. Of the phosphoinositide specific phospholipase C enzymes, C beta is regulated by heterotrimeric G protein coupled receptors, while the closely related C gamma 1 and C gamma 2 enzymes are controlled by receptor tyrosine kinases. The C gamma 1 and C gamma 2 enzymes are composed of phospholipase domains that flank regions of homology to noncatalytic domains of the SRC oncogene product, SH2 and SH3.
The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. It is a crucial enzyme in transmembrane signaling.
Interacts (via SH2 domain) with CSF1R (tyrosine phosphorylated).
Phosphorylated on tyrosine residues by CSF1R. Phosphorylated on tyrosine residues by BTK and SYK; upon ligand-induced activation of a variety of growth factor receptors and immune system receptors. Phosphorylation leads to increased phospholipase activity.
Defects in PLCG2 are the cause of familial cold autoinflammatory syndrome type 3 (FCAS3) [MIM:614468]. An autosomal dominant immune disorder characterized by the development of cutaneous urticaria, erythema, and pruritis in response to cold exposure. Affected individuals have variable additional immunologic defects, including antibody deficiency, decreased numbers of B cells, defective B cells, increased susceptibility to infection, and increased risk of autoimmune disorders.
Contains 1 C2 domain.
Contains 1 PH domain.
Contains 1 PI-PLC X-box domain.
Contains 1 PI-PLC Y-box domain.
Contains 2 SH2 domains.
Contains 1 SH3 domain.
Entrez Gene: 5336 Human
Entrez Gene: 234779 Mouse
Entrez Gene: 29337 Rat
Omim: 600220 Human
SwissProt: P16885 Human
SwissProt: Q8CIH5 Mouse
SwissProt: P24135 Rat
Unigene: 413111 Human
Unigene: 192699 Mouse
Unigene: 9751 Rat
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications