Rabbit Anti-phospho-RAD51(Tyr315) antibody
|别 名||RAD51(phospho Y315); BRCA1/BRCA2 containing complex, subunit 5; BRCC 5; BRCC5; DNA repair protein RAD51 homolog 1; DNA repair protein rhp51; E coli RecA homolog; HGNC:9817; Homolog of E coli RecA; homolog of S cerevisiae RAD51; HRAD51; HsRad51; HsT16930; Rad 51; RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae); RAD51 homolog; RAD51 homolog S. cerevisiae; RAD51 S cerevisiae homolog; RAD51A; RECA; RecA homolog E. coli; RecA like protein; RecA, E. coli, homolog of; recombination protein A.|
|研究领域||肿瘤 免疫学 信号转导 转录调节因子|
|交叉反应||Human, Mouse, Rat,|
|产品应用||WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
|分 子 量||37kDa|
|性 状||Lyophilized or Liquid|
|免 疫 原||KLH conjugated Synthesised phosphopeptide derived from human RAD51 around the phosphorylation site of Tyr315:KI(p-Y)DS|
|纯化方法||affinity purified by Protein A|
|储 存 液||0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.|
|保存条件||Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.|
The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with the ssDNA-binding protein RPA and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. This protein is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis. Two alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported. Transcript variants utilizing alternative polyA signals exist.
Participates in a common DNA damage response pathway associated with the activation of homologous recombination and double-strand break repair. Binds to single and double stranded DNA and exhibits DNA-dependent ATPase activity. Underwinds duplex DNA and forms helical nucleoprotein filaments. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3.
Nucleus. Cytoplasm. Cytoplasm, perinuclear region. Mitochondrion matrix. Note=Colocalizes with RAD51AP1 and RPA2 to multiple nuclear foci upon induction of DNA damage. DNA damage induces an increase in nuclear levels.
Highly expressed in testis and thymus, followed by small intestine, placenta, colon, pancreas and ovary. Weakly expressed in breast.
Phosphorylated. Phosphorylation of Thr-309 by CHEK1 may enhance association with chromatin at sites of DNA damage and promote DNA repair by homologous recombination. Phosphorylation by ABL1 inhibits function.
Defects in RAD51 are a cause of susceptibility to breast cancer (BC) [MIM:114480]. A common malignancy originating from breast epithelial tissue. Breast neoplasms can be distinguished by their histologic pattern. Invasive ductal carcinoma is by far the most common type. Breast cancer is etiologically and genetically heterogeneous. Important genetic factors have been indicated by familial occurrence and bilateral involvement. Mutations at more than one locus can be involved in different families or even in the same case.
Belongs to the RecA family. RAD51 subfamily.
Contains 1 HhH domain.
Entrez Gene: 5888 Human
Entrez Gene: 19361 Mouse
Entrez Gene: 499870 Rat
Omim: 179617 Human
SwissProt: Q06609 Human
SwissProt: Q08297 Mouse
Unigene: 631709 Human
Unigene: 330492 Mouse
Unigene: 20474 Rat
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.