Rabbit Anti-phospho-Cdc25B (Ser149) antibody
|别 名||Cdc25B (Phospho-Ser149); Cdc25B (Phospho-S149); p-Cdc25B (Ser149); p-Cdc25B (S149); Cdc 25B; Cdc25b; Cdc-25b; CDC25HU2; Cdc25m2; Cell division cycle 25 homolog B; Cell division cycle 25B; Cell division cycle 25B isoform 1; Cell division cycle 25B isoform 2; Cell division cycle 25B isoform 3; Cell division cycle 25B isoform 4; Cell division cycle 25B isoform 5; Dual specificity phosphatase Cdc25B; M phase inducer phosphatase 2; M-phase inducer phosphatase 2; MPIP2_HUMAN.|
|研究领域||肿瘤 细胞生物 免疫学 细胞周期蛋白 激酶和***酸酶 表观遗传学|
|交叉反应||Human, Mouse, Rat,|
|产品应用||WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:50-200 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
|分 子 量||65kDa|
|性 状||Lyophilized or Liquid|
|免 疫 原||KLH conjugated synthesised phosphopeptide derived from human Cdc25B around the phosphorylation site of Ser149:FR(p-S)LP|
|纯化方法||affinity purified by Protein A|
|储 存 液||0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.|
|保存条件||Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.|
CDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist. [provided by RefSeq, Jul 2008].
Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity.
Interacts with MAPK14 and 14-3-3 proteins.
Cytoplasm, cytoskeleton, centrosome. Cytoplasm, cytoskeleton, spindle pole.
Phosphorylated by BRSK1 in vitro. Phosphorylated by CHEK1, which inhibits the activity of this protein. Phosphorylation at Ser-353 by AURKA might locally participate in the control of the onset of mitosis. Phosphorylation by MELK at Ser-169 promotes localization to the centrosome and the spindle poles during mitosis. Phosphorylation at Ser-323 and Ser-375 by MAPK14 is required for binding to 14-3-3 proteins.
Belongs to the MPI phosphatase family.
Contains 1 rhodanese domain.
Entrez Gene: 994 Human
Entrez Gene: 12531 Mouse
Entrez Gene: 171103 Rat
Omim: 116949 Human
SwissProt: P30305 Human
SwissProt: P30306 Mouse
SwissProt: P48966 Rat
Unigene: 153752 Human
Unigene: 38444 Mouse
Unigene: 11312 Rat
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applicatio