Rabbit Anti-phospho-ERK5 (Thr218 + Tyr220) antibody
|英文名称||phospho-ERK5 (Thr218 + Tyr220)|
|别 名||ERK5 (phospho T218 + Y220); ERK5 (phospho Thr218 + Tyr220); Big MAP kinase 1; BMK 1; BMK 1 kinase; BMK-1; BMK1; BMK1 Kinase; EC 126.96.36.199; ERK 4; ERK 5; ERK4; Extracellular Signal Regulated Kinase 5; Extracellular signal-regulated kinase 5; MAP kinase 7; MAPK 7; MAPK7; Mitogen Activated Protein Kinase 7; Mitogen-activated protein kinase 7; MK07_HUMAN; OTTHUMP00000065906; OTTHUMP00000065907; PRKM 7; PRKM7; PROTEIN KINASE, MITOGEN-ACTIVATED, 7.|
|研究领域||肿瘤 细胞生物 信号转导 转录调节因子 激酶和***酸酶|
|交叉反应||Human, Mouse, Rat, Dog, Pig, Cow, Sheep, Guinea Pig,|
|产品应用||ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
|分 子 量||90kDa|
|性 状||Lyophilized or Liquid|
|免 疫 原||KLH conjugated synthesised phosphopeptide derived from human ERK5 around the phosphorylation site of Thr218 + Tyr220 [FM(p-T)E(p-Y)VA]:FM(p-T)E(p-Y)VA|
|纯化方法||affinity purified by Protein A|
|储 存 液||0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.|
|保存条件||Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.|
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is specifically activated by mitogen-activated protein kinase kinase 5 (MAP2K5/MEK5). It is involved in the downstream signaling processes of various receptor molecules including receptor type kinases, and G protein-coupled receptors. In response to extracelluar signals, this kinase translocates to cell nucleus, where it regulates gene expression by phosphorylating, and activating different transcription factors. Four alternatively spliced transcript variants of this gene encoding two distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
Plays a role in various cellular processes such as proliferation, differentiation and cell survival. The upstream activator of MAPK7 is the MAPK kinase MAP2K5. Upon activation, it translocates to the nucleus and phosphorylates various downstream targets including MEF2C. EGF activates MAPK7 through a Ras-independent and MAP2K5-dependent pathway. May have a role in muscle cell differentiation. May be important for endothelial function and maintenance of blood vessel integrity. MAP2K5 and MAPK7 interact specifically with one another and not with MEK1/ERK1 or MEK2/ERK2 pathways.
Interacts with MAP2K5. Forms oligomers. Interacts with MEF2A, MEF2C and MEF2D; the interaction phosphorylates the MEF2s and enhances transcriptional activity of MEF2A, MEF2C but not MEF2D. Interacts with SGK1.
Cytoplasm. Nucleus. Note=Translocates to the nucleus upon activation..
Expressed in many adult tissues. Abundant in heart, placenta, lung, kidney and skeletal muscle. Not detectable in liver.
Dually phosphorylated on Thr-219 and Tyr-221, which activates the enzyme. Autophosphorylated in vitro on threonine and tyrosine residues when the C-terminal part of the kinase, which could have a regulatory role, is absent.
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. Contains 1 protein kinase domain.
Entrez Gene: 5598 Human
Entrez Gene: 23939 Mouse
Entrez Gene: 114509 Rat
Omim: 602521 Human
SwissProt: Q13164 Human
SwissProt: Q9WVS8 Mouse
SwissProt: P0C865 Rat
Unigene: 150136 Human
Unigene: 38172 Mouse
Unigene: 144629 Rat
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications