Rabbit Anti-phospho-beta Adducin (Ser713) antibody
|英文名称||phospho-beta Adducin (Ser713)|
|别 名||beta Adducin (phospho S713); p-beta Adducin (phospho S713); phospho-alpha Adducin(Ser726); phospho-gamma Adducin(Ser693); ADD1; ADD2; ADD3; ADDA; ADDB; ADDL; Adducin 1 (alpha); Adducin 1; Adducin 2 (beta); Adducin 2; Adducin 3 (gamma); Adducin 3; Adducin like; Alpha adducin; Beta adducin.|
|研究领域||细胞生物 信号转导 细胞类型标志物 细胞骨架|
|交叉反应||Human, Mouse, Rat, Chicken, Zebrafish,|
|产品应用||WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
|分 子 量||80kDa|
|性 状||Lyophilized or Liquid|
|免 疫 原||KLH conjugated synthesised phosphopeptide derived from human beta Adducin around the phosphorylation site of Ser713:TP(p-S)FL|
|纯化方法||affinity purified by Protein A|
|储 存 液||0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.|
|保存条件||Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.|
Adducins are a family of cytoskeletal proteins encoded by three genes (alpha, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. Alpha and beta adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Alpha and gamma adducins are ubiquitously expressed. In contrast, beta adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca2+/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms; however, not all variants have been fully described.
Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin. Calmodulin binds preferentially to the beta subunit.
Heterodimer of an alpha and a beta subunit.
Expressed mainly in brain, spleen, kidney cortex and medulla, and heart. Also expressed in human umbilical vein endothelial cells, human vascular smooth muscle cells, kidney tubular cells and K-562 cell line.
The N-terminus is blocked.
Belongs to the aldolase class II family. Adducinbn subfamily.
Entrez Gene: 119 Human
Entrez Gene: 11519 Mouse
Entrez Gene: 24171 Rat
Omim: 102681 Human
SwissProt: P35612 Human
SwissProt: Q9QYB8 Mouse
SwissProt: Q05764 Rat
Unigene: 188528 Human
Unigene: 11353 Rat
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications