磷酸化真核延伸因子激酶2抗体
产品名称： 磷酸化真核延伸因子激酶2抗体
英文名称： Phospho-EEF2k (Ser359)
产品编号： hz-13058R
产品价格： null
产品产地： 中国/上海
品牌商标： HZbscience
更新时间： 2023-08-17T10:24:20
使用范围： WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500
上海沪震实业有限公司
- 联系人 : 鲍丽雯
- 地址 : 上海市闵行区闵北路88弄1-30号第22幢AQ136室
- 邮编 : 200612
- 所在区域 : 上海
- 电话 : 139****0749
- 传真 : 021-60345367
- 邮箱 : www.shzbio.net
Rabbit Anti-Phospho-EEF2k (Ser359) antibody
| 产品编号 | hz-13058R |
| 英文名称 | Phospho-EEF2k (Ser359) |
| 中文名称 | 磷酸化真核延伸因子激酶2抗体 |
| 别 名 | EEF2K (phospho S359); p-EEF2K (phospho S359); Calcium/calmodulin dependent eukaryotic elongation factor 2; Calcium/calmodulin dependent eukaryotic elongation factor 2 kinase; Calmodulin dependent protein kinase III; cb365; eEF 2 kinase; eEF 2K; EEF2K protein; Elongation factor 2 kinase; Eukaryotic elongation factor 2 kinase; kinase eEF2K; SMEF2K; EF2K_HUMAN. |
| 产品类型 | 磷酸化抗体 |
| 研究领域 | 肿瘤 细胞生物 信号转导 细胞凋亡 转录调节因子 激酶和磷酸酶 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Chicken, Dog, Horse, Rabbit, Sheep, |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 82kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human EEF2k around the phosphorylation site of Ser359:CG(p-S)P |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| PubMed | PubMed |
| 产品介绍 | background: Eukaryotic elongation factor 2 kinase (EEF2k) previously known as Ca2+/calmodulin dependent protein kinase III, is an abundant cytoplasmic protein highly specific for elongation factor 2 (eEf2). Phosphorylation of eEF2 by eEF2 kinase on specific threonine residues results in the inactivation of eEF-2 and in termination of mRNA translation. The activity of eEF2 kinase is not only dependent upon Ca2+ ions, calmodulin (CaM) and insulin, but is also regulated both negatively and positively via phosphorylation by different protein kinases (AMPK, S6K1, p90 RSK). There is also evidence that eEF-2 phosphorylation is involved in the regulation of cell cycle progression, cellular differentiation, oogensis and malignant tumors. Function: Threonine kinase that regulates protein synthesis bycontrolling the rate of peptide chain elongation. Upon activationby a variety of upstream kinases including AMPK or TRM7,phosphorylates the elongation factor EEF2 at a single site, rendersit unable to bind ribosomes and thus inactive. In turn, the rate ofprotein synthesis is reduced. Subunit: Monomer or homodimer. Post-translational modifications: Autophosphorylated at multiple residues, Thr-348 being themajor site. Phosphorylated by AMP-activated protein kinase AMPK atSer-398 leading to EEF2K activation and protein synthesisinhibition. Phosphorylated by TRPM7 at Ser-78 resulting in improvedprotein stability, higher EE2F phosphorylated and subsequentlyreduced rate of protein synthesis. Phosphorylation by other kinasessuch as CDK1 and MAPK13 at Ser-359 or RPS6KA1 and RPS6KB1 atSer-366 instead decrease EEF2K activity and promote proteinsynthesis. Similarity: Belongs to the protein kinase superfamily. Alpha-type protein kinase family. Contains 1 alpha-type protein kinase domain. SWISS: O00418 Gene ID: 101930123 Database links: UniProtKB/Swiss-Prot: O00418.2 Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications |