Rabbit Anti-phospho-NFAT4 (Ser165) antibody
|别 名||NFAT4 (phospho S165); p-NFAT4 (phospho S165); C80703; cytoplasmic 3; D8Ertd281e; NF AT4; NF ATc3; NF-AT4; NF-ATc3; NFAC3_HUMAN; NFATc3; NFATx; Nuclear factor of activated T cells, cytoplasmic 3; nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 3; Nuclear factor of activated T-cells; T cell transcription factor NFAT4; T-cell transcription factor NFAT4.|
|研究领域||细胞生物 信号转导 自然杀伤细胞 t-淋巴细胞 表观遗传学|
|交叉反应||Human, Mouse, Chicken, Cow, Horse, Rabbit, Sheep,|
|产品应用||WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
|分 子 量||116kDa|
|性 状||Lyophilized or Liquid|
|免 疫 原||KLH conjugated synthesised phosphopeptide derived from human NFAT4 around the phosphorylation site of Ser165:SP(p-S)PA|
|纯化方法||affinity purified by Protein A|
|储 存 液||0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.|
|保存条件||Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.|
The product of this gene is a member of the nuclear factors of activated T cells DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation and an inducible nuclear component. Other members of this family participate to form this complex also. The product of this gene plays a role in the regulation of gene expression in T cells and immature thymocytes. Several transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Nov 2010]
Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2.
Cytoplasm. Nucleus. Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription.
Isoform 1 is predominantly expressed in thymus and is also found in peripheral blood leukocytes and kidney. Isoform 2 is predominantly expressed in skeletal muscle and is also found in thymus, kidney, testis, spleen, prostate, ovary, small intestine, heart, placenta and pancreas. Isoform 3 is expressed in thymus and kidney. Isoform 4 is expressed in thymus and skeletal muscle.
Phosphorylated by NFATC-kinase; dephosphorylated by calcineurin.
Contains 1 RHD (Rel-like) domain.
Entrez Gene: 4775 Human
Entrez Gene: 18021 Mouse
Entrez Gene: 361400 Rat
Omim: 602698 Human
SwissProt: Q12968 Human
SwissProt: P97305 Mouse
Unigene: 436585 Human
Unigene: 383185 Mouse
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications