Rabbit Anti-phospho-PKC delta (Ser299) antibody
|英文名称||phospho-PKC delta (Ser299)|
|别 名||PKC delta (phospho S299); p-PKC delta (phospho S299); CVID9; D14Ertd420e; Kinase PKC delta; KPCD; KPCD_HUMAN; MAY 1; MAY1; MGC49908; nPKC delta; nPKC-delta; PCKd; PKC d; PKC delta; PKCd; PKCD; PKCdelta; PRKC D; PRKC delta; Prkcd; Protein Kinase C delta; Protein kinase C delta type; Protein kinase C delta VIII; Protein Kinase Cdelta; Tyrosine protein kinase PRKCD.|
|研究领域||肿瘤 细胞生物 神经生物学 信号转导 激酶和***酸酶|
|产品应用||WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
|分 子 量||77kDa|
|性 状||Lyophilized or Liquid|
|免 疫 原||KLH conjugated synthesised phosphopeptide derived from human PKC delta around the phosphorylation site of Ser299:RA(p-S)RR|
|纯化方法||affinity purified by Protein A|
|储 存 液||0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.|
|保存条件||Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.|
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. The protein encoded by this gene is one of the PKC family members. Studies both in human and mice demonstrate that this kinase is involved in B cell signaling and in the regulation of growth, apoptosis, and differentiation of a variety of cell types. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008]
This is calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. May play a role in antigen-dependent control of B-cell function.
Phosphorylates MUC1 in the C-terminal and regulates the interaction between MUC1 and beta-catenin.
Phosphorylated on Thr-507, within the activation loop. Autophosphorylated and/or phosphorylated. Although the Thr-507 phosphorylation occurs it is not a prerequisite for enzymatic activity.
Belongs to the protein kinase superfamily.
AGC Ser/Thr protein kinase family.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 C2 domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
Contains 1 protein kinase domain.
Entrez Gene: 5580 Human
Entrez Gene: 18753 Mouse
Entrez Gene: 170538 Rat
Omim: 176977 Human
SwissProt: Q05655 Human
SwissProt: P28867 Mouse
SwissProt: P09215 Rat
Unigene: 155342 Human
Unigene: 2314 Mouse
Unigene: 98279 Rat
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications