Rabbit Anti-Phospho-PERK(Thr980) antibody
|别 名||p-PERK(Thr980); PERK(Phospho Thr980); PERK(Phospho T980); HRI; HsPEK; Pancreatic eIF2-alpha kinase; PEK; PRKR like endoplasmic reticulum kinase; WRS; DKFZp781H1925; EC 220.127.116.11; EIF2AK3; Eukaryotic translation initiation factor 2 alpha kinase 3; Heme regulated EIF2 alpha kinase. p-PERK(Thr982); PERK(Phospho Thr982); PERK(Phospho T982);|
|研究领域||肿瘤 细胞生物 免疫学 信号转导 细胞凋亡 转录调节因子 激酶和***酸酶|
|交叉反应||Human, Mouse, Rat, Rabbit,|
|产品应用||ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
|分 子 量||119kDa|
|性 状||Lyophilized or Liquid|
|免 疫 原||KLH conjugated synthetic peptide derived from mouse Phospho-PERK(Thr980):H(p-T)GQ|
|纯化方法||affinity purified by Protein A|
|储 存 液||0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.|
|保存条件||Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.|
The protein encoded by this gene phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. It is a type I membrane protein located in the endoplasmic reticulum (ER), where it is induced by ER stress caused by malfolded proteins. Mutations in this gene are associated with Wolcott-Rallison syndrome. [provided by RefSeq, Jan 2010].
Phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation and thus to a rapid reduction of translational initiation and repression of global protein synthesis. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin D1.
Forms dimers with HSPA5/BIP in resting cells. Oligomerizes in ER-stressed cells. Interacts with DNAJC3.
Endoplasmic reticulum membrane; Single-pass type I membrane protein.
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.
Contains 1 protein kinase domain.
Entrez Gene: 9451 Human
Entrez Gene: 13666 Mouse
Entrez Gene: 29702 Rat
Omim: 604032 Human
SwissProt: Q9NZJ5 Human
SwissProt: Q9Z2B5 Mouse
SwissProt: Q9Z1Z1 Rat
Unigene: 591589 Human
Unigene: 247167 Mouse
Unigene: 24897 Rat
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications