Mouse Anti-alpha Tubulin (Acetyl Lys40) antibody
|英文名称||alpha Tubulin (Acetyl Lys40)|
|别 名||Alpha tubulin 1; Alpha-tubulin 1; Detyrosinated alpha Tubulin; FLJ30169; H2 alpha; TBA4A_HUMAN; Testis specific alpha tubulin; Testis-specific alpha-tubulin; TUBA 4A; TUBA1; Tuba4a; Tubulin alpha 1 (testis specific); Tubulin alpha 1; Tubulin alpha 1 chain; Tubulin alpha 4a; Tubulin alpha 4A chain; Tubulin alpha-1 chain; Tubulin alpha-4A chain; Tubulin H2 alpha; Tubulin H2-alpha; TUBA4A. α tubulin;|
|研究领域||细胞生物 信号转导 细胞骨架|
|克 隆 号||6B5|
|交叉反应||Human, Mouse, Rat, Danio rerio,|
|产品应用||WB=1:500-1000 ELISA=1:500-1000 IHC-P=1:200-500 IHC-F=1:200-500 ICC=1:100-500 IF=1:200-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
|分 子 量||50kDa|
|免 疫 原||KLH conjugated synthetic peptide derived from human alpha Tubulin (Acetyl Lys40):|
|纯化方法||affinity purified by Protein G|
|储 存 液||0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.|
|保存条件||Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.|
Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. This gene encodes an alpha tubulin that is a highly conserved homolog of a rat testis-specific alpha tubulin. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2013]
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain
Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells.
Widely expressed. Overexpressed in Platelet, Brain, and Frontal cortex
Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules (Probable).
Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling (By similarity).
Belongs to the tubulin family.
Entrez Gene: 7277 Human
Entrez Gene: 22145 Mouse
Entrez Gene: 316531 Rat
Omim: 191110 Human
SwissProt: P68366 Human
SwissProt: P68368 Mouse
SwissProt: Q5XIF6 Rat
Unigene: 75318 Human
Unigene: 1155 Mouse
Unigene: 92961 Rat
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications