Rabbit Anti-Ephexin-1/FITC Conjugated antibody
|别 名||ARHGEF27; Eph-interacting exchange protein; EPHEXIN; Ephexin 1; Neuronal guanine nucleotide exchange factor; Ngef; NGEF_HUMAN.|
|规格价格||100ul/2980元 购买 大包装/询价|
|说 明 书||100ul|
|研究领域||细胞生物 神经生物学 信号转导 G蛋白偶联受体 G蛋白信号|
|交叉反应||Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse,|
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
|分 子 量||82kDa|
|性 状||Lyophilized or Liquid|
|免 疫 原||KLH conjugated synthetic peptide derived from human EPHEXIN (451-550aa)|
|纯化方法||affinity purified by Protein A|
|储 存 液||0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.|
|保存条件||Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.|
EPHEXIN is a 710 amino acid protein that localizes to both the membrane and the cytoplasm and contains one SH3 domain, one PH domain and one DH domain. Expressed at high levels in brain and present at lower levels in lung tissue, EPHEXIN interacts with EphA4 and functions as a guanine nucleotide exchange factor (GEF) that is capable of activating Rho A, Rac 1 and Cdc42 and is thought to play a role in axon guidance and growth cone collapse. EPHEXIN is subject to Src-dependent phosphorylation, an event that increases the GEF activity of EPHEXIN toward Rho A. Human EPHEXIN, which exists as multiple alternatively spliced isoforms, shares a high degree of sequence homology with its mouse counterpart, suggesting a conserved role between species.
Acts as a guanine nucleotide exchange factor (GEF) which differentially activates the GTPases RHOA, RAC1 and CDC42. Plays a role in axon guidance regulating ephrin-induced growth cone collapse. Upon activation by ephrin through EPHA4, the GEF activity switches toward RHOA resulting in its activation. Activated RHOA promotes cone retraction at the expense of RAC1-and CDC42-stimulated growth cone extension.
Interacts with CDK5R1 and EPHA4; activated by EPHA4 through the CDK5 kinase
Cytoplasm. Membrane. Cell projection, growth cone. Associated with membranes. Localizes to axonal growth cones.
Highly expressed in brain specifically in caudate nucleus and to a lower extent in amygdala and hippocampus. Also detected in lung.
Src-dependent phosphorylation at Tyr-179 upon EPHA4 activation increases the guanine exchange factor activity toward RHOA.
Contains 1 DH (DBL-homology) domain.
Contains 1 PH domain.
Contains 1 SH3 domain.
Entrez Gene: 25791 Human
Entrez Gene: 53972 Mouse
Entrez Gene: 246217 Rat
Entrez Gene: 424744 Chicken
Omim: 605991 Human
SwissProt: Q8N5V2 Human
SwissProt: Q8CHT1 Mouse
SwissProt: Q5BKC9 Rat
Unigene: 97316 Human
Unigene: 435439 Mouse
Unigene: 20670 Rat
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications
Epxin是定位于膜和细胞质中的710个氨基酸蛋白，包含一个SH3结构域、一个pH-结构域和一个DH结构域。在高水平的脑中表达，并在肺组织中存在较低水平，视黄蛋白与EPHA4相互作用，并作为能激活Rho A、Rac 1和CDC42的鸟嘌呤核苷酸交换因子（GEF）发挥作用，并被认为在轴突导向和生长锥崩溃中起作用。视素依赖于Src依赖的***酸化，这是一种事件，它使Epxin的GEF活性增加到Rho。A，人啡肽，作为多个交替剪接的亚型存在，与它的小鼠对应物具有高度的序列同源性，暗示SP之间的保守作用。爱西斯。