Rabbit Anti-Exportin 1/FITC Conjugated antibody
|别 名||Chromosome region maintenance 1 protein homolog; CRM 1; CRM1; CRM1 homolog; DKFZp686B1823; Exp 1; Exp-1;Exp1; Exportin 1; Exportin1; Exportin-1; XPO 1; XPO1; XPO1_HUMAN.|
|规格价格||100ul/2980元 购买 大包装/询价|
|说 明 书||100ul|
|研究领域||细胞生物 染色质和核信号 信号转导 转录调节因子|
|交叉反应||Human, Mouse, Rat, Pig,|
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
|分 子 量||118kDa|
|性 状||Lyophilized or Liquid|
|免 疫 原||KLH conjugated synthetic peptide derived from human Exportin 1|
|纯化方法||affinity purified by Protein A|
|储 存 液||0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.|
|保存条件||Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.|
CRM1 (chromosome region maintenance 1 protein homolog) mediates the nuclear export of cellular proteins (cargoes) bearing a leucine-rich nuclear export signal (NES) and of RNAs. In the nucleus, in association with RANBP3, CRM1 binds cooperatively to the NES on its target protein and to the GTPase Ran in its active GTP-bound form. Docking of this complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the cargo from the export receptor. The direction of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. CRM1 also mediates the nuclear export of the Rev protein of HIV-1 and Rex protein of HTLV-1. It is involved in Rex dimerization, U3 snoRNA transport from Cajal bodies to nucleoli.
CRM1 is also involved in the control of several cellular processes by controlling the localization of cyclin B, MPAK, and MAPKAP kinase 2. CRM1 is also involved in the regulation NFAT and AP-1.
Found in a U snRNA export complex with RNUXA/PHAX, NCBP1, NCBP2, RAN, XPO1 and m7G-capped RNA (By similarity). Component of a nuclear export receptor complex composed of KPNB1, RAN, SNUPN and XPO1. Found in a trimeric export complex with SNUPN, RAN and XPO1. Found in a nuclear export complex with RANBP3 and RAN. Found in a 60S ribosomal subunit export complex with NMD3, RAN, XPO1. Interacts with DDX3X, NMD3, NUPL2, NUP88, NUP214, RANBP3 and TERT. Also found in complex with several viral proteins involved in RNAs' nuclear export like HIV-1 Rev and HTLV-1 Rex. Interacts presumably with influenza A nucleoprotein. Interacts with Epstein-Barr virus BMLF1. Interacts with NEMF (via its N-terminus). Interacts with the monomeric form of BIRC5/survivin deacetylated at 'Lys-129'.
Cytoplasm. Nucleus, nucleoplasm. Nucleus, Cajal body. Nucleus, nucleolus. Note=Located in the nucleoplasm, Cajal bodies and nucleoli. Shuttles between the nucleus/nucleolus and the cytoplasm.
Expressed in heart, brain, placenta, lung, liver, skeletal muscle, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes. Not expressed in the kidney.
Belongs to the exportin family.
Contains 10 HEAT repeats.
Contains 1 importin N-terminal domain.
Entrez Gene: 7514 Human
Entrez Gene: 103573 Mouse
Entrez Gene: 85252 Rat
Omim: 602559 Human
SwissProt: O14980 Human
SwissProt: Q6P5F9 Mouse
SwissProt: Q80U96 Rat
Unigene: 370770 Human
Unigene: 217547 Mouse
Unigene: 34087 Rat
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications
CRM1（染色体区域维持1蛋白同源物）介导具有富含亮氨酸的核输出信号（NES）和RNA的细胞蛋白（货物）的核输出。在核中，与RANBP3相关联的CRM1在其靶蛋白上与NES协同结合，并且GTP酶在其活性的GTP结合形式中结合。这种复合物与核孔复合体（NPC）的对接是通过结合核孔蛋白介导的。在将核出口复合物转运到细胞质中时，将复合物和RAN-GTP的水解拆分为RAN GDP（分别由RANBP1和RangAP1诱导）导致货物从出口受体释放。核出口的方向被认为是由细胞质和细胞核之间的GTP和GDP结合形式的RAN的不对称分布所赋予的。CRM1还介导HIV-1的Rev蛋白和HTLV-1的雷克斯蛋白的核输出。它参与了雷克斯二聚反应，U3 SnRNA从Cajal体向核仁的转运。