Rabbit Anti-ADAMTS5/FITC Conjugated antibody
|别 名||ADAMTS5; A disintegrin and metalloproteinase with thrombospondin motifs 5; A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 5; A Disintigrin And Metalloproteinase with ThromboSpondin motif-5; ADAM metallopeptidase with thrombospondin type 1 motif 5; ADAM TS 11; ADAM TS 5; ADAM TS5; ADAMTS 11; ADAMTS 5; ADAMTS11; ADMP 2; ADMP2; Aggrecanase 2; aggrecanase-2; FLJ36738; Implantin; ThromboSpondin motif-5.|
|规格价格||100ul/2980元 购买 大包装/询价|
|说 明 书||100ul|
|研究领域||肿瘤 免疫学 激酶和***酸酶|
|交叉反应||Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit,|
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
|分 子 量||102kDa|
|性 状||Lyophilized or Liquid|
|免 疫 原||KLH conjugated synthetic peptide derived from human Aggrecan 2|
|纯化方法||affinity purified by Protein A|
|储 存 液||0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.|
|保存条件||Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.|
ADAMTS5 was first described as ADAMTS5, a protein elevated in mice during the peri-implantion period. At the same time, another group identified Aggrecanase 11, a protein elevated in arthritic synovium. The name was later changed to ADAMTS5. ADAMTS5 is expressed in human and mouse. It has been found in heart, lung, cervix, uterus, ovary, brain, cartilage, and numerous other tissues, as well as chondroblastoma cell lines. Initial observations indicated a role for ADAMTS5 in aggrecan cleavage and cartilage destruction, especially in arthritis, and potentially a role in embryo implantation. ADAMTS5 contains the canonical HExxHxxxxxH zinc metalloproteinase motif, and has been shown to efficiently cleave Aggrecan. In addition to the metalloprotease domain, ADAMTS5 has a propeptide domain, a prohormone convertase (PC, furin) cleavage site, a cysteine-rich domain, a spacer domain, and two thrombospondin-1 like domains. ADAMTS5 is inhibited by the endogenous MMP inhibitors (TIMP1, 2, 3, and 4) but most efficiently by TIMP3. Unlike many of the ADAMs proteases, ADAMTS5 does not have a transmembrane domain, and is a secreted protein. Full length ADAMTS5 is a 930 amino acid protein with a predicted molecular mass is 101.7 kDa, but glycosylation and the abundance of cysteine residues gives ADAMTS5 a greater apparent molecular weight on reduced SDS PAGE gels. When ADAMTS5 is secreted, it is cleaved at the furin cleavage site (predicted molecular mass 73.2 kDa) and then further cleaved to generate a range of smaller forms.
Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. May play an important role in the destruction of aggrecan in arthritic diseases. May play a role in proteolytic processing mostly during the peri-implantation period.
Secreted, extracellular space, extracellular matrix.
Expressed at low level in placenta primarily but also detected in heart and brain, cervix, uterus, bladder, esophagus, rib cartilage, chondroblastoma, fibrous tissue and a joint capsule from an arthritic patient.
The precursor is cleaved by a furin endopeptidase.
C- and O-glycosylated. O-fucosylated by POFUT2 on a serine or a threonine residue found within the consensus sequence C1-X(2)-(S/T)-C2-G of the TSP type-1 repeat domains where C1 and C2 are the first and second cysteine residue of the repeat, respectively. Fucosylated repeats can then be further glycosylated by the addition of a beta-1,3-glucose residue by the glucosyltransferase, B3GALTL. Fucosylation can mediate the efficient secretion of ADAMTS family members. Can be C-glycosylated with one or two mannose molecules on tryptophan residues within the consensus sequence W-X-X-W of the TPRs, and N-glycosylated. These other glycosylations can also facilitate secretion.
Glycosylated. Can be O-fucosylated by POFUT2 on a serine or a threonine residue found within the consensus sequence C1-X(2)-(S/T)-C2-G of the TSP type-1 repeat domains where C1 and C2 are the first and second cysteine residue of the repeat, respectively. Fucosylated repeats can then be further glycosylated by the addition of a beta-1,3-glucose residue by the glucosyltransferase, B3GALTL. Fucosylation mediates the efficient secretion of ADAMTS family members. Also can be C-glycosylated with one or two mannose molecules on tryptophan residues within the consensus sequence W-X-X-W of the TPRs, and N-glycosylated. These other glycosylations can also facilitate secretion.
Contains 1 disintegrin domain.
Contains 1 peptidase M12B domain.
Contains 2 TSP type-1 domains.
Entrez Gene: 11096 Human
Entrez Gene: 23794 Mouse
Entrez Gene: 304135 Rat
Omim: 605007 Human
SwissProt: Q9UNA0 Human
SwissProt: Q9R001 Mouse
Unigene: 58324 Human
Unigene: 112933 Mouse
Unigene: 437478 Mouse
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications
ADAMTS5最初被描述为ADAMTS5，一种在小鼠围手术期升高的蛋白。同时，另一组鉴定了在关节炎滑膜中升高的蛋白AgGraceA酶11。该名称后来改为ADAMTS5。ADAMTS5在人和小鼠中均有表达。它已经在心脏、肺、宫颈、子宫、卵巢、脑、软骨和许多其他组织中发现，以及软骨母细胞瘤细胞系。初步观察表明ADAMTS5在聚集蛋白断裂和软骨破坏中的作用，特别是在关节炎中，并且可能在胚胎着床中起作用。ADAMTS5含有典型的HOXXHXXXXH锌金属蛋白酶基序，已被证明能有效切割聚集蛋白聚糖。除了金属蛋白酶结构域，ADAMTS5具有前肽结构域、前***转化酶（PC、Furin）切割位点、富含半胱氨酸的结构域、间隔域和两个血小板反应素-1样结构域。ADAMTS5被内源性MMP抑制剂（TIMP1、2, 3和4）抑制，但最有效的是TIMP3。与许多亚当斯蛋白酶不同，ADAMTS5不具有跨膜结构域，并且是分泌蛋白。全长ADAMTS5是一个930氨基酸的蛋白质，其预测的分子量为101.7 kDa，但糖基化和半胱氨酸残基的丰度使ADAMTS5在SDS-PAGE凝胶上具有更大的表观分子量。当分泌ADAMTS5时，它在呋喃裂解位点（预测的分子量为73.2 kDa）处裂解，然后进一步裂解以产生较小的形式的范围。