Rabbit Anti-Phospho-Smad2(Ser465 + Ser467)/FITC Conjugated antibody
|英文名称||Anti-Phospho-Smad2(Ser465 + Ser467)/FITC|
|别 名||Smad2(Phospho S465 + S467); phospho-Smad2(p-Ser465/467); p-Smad2(Ser465/467); phospho-Smad2(p-S465/467); Smad2 (phospho S465 + S467); p-Smad2 (phospho S465 + S467); hMAD 2; hSMAD2; JV18 1; JV18; JV181; MAD; MAD Related Protein 2; MADH2; MADR2; MGC22139; MGC34440; Mothers Against Decapentaplegic Homolog 2; mothers against DPP homolog 2; SMAD 2; SMAD; SMAD2; SMAD2_HUMAN.|
|规格价格||100ul/2980元 购买 大包装/询价|
|说 明 书||100ul|
|研究领域||免疫学 信号转导 细胞凋亡 转录调节因子|
|交叉反应||Human, Mouse, Rat, Chicken, Dog, Cow, Horse,|
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
|分 子 量||58kDa|
|性 状||Lyophilized or Liquid|
|免 疫 原||KLH conjugated Synthesised phosphopeptide derived from human SMAD2 around the phosphorylation site of Ser465/467|
|纯化方法||affinity purified by Protein A|
|储 存 液||0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.|
|保存条件||Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.|
Smad2 is a 58 kDa member of a family of proteins involved in cell proliferation, differentiation and development. The Smad family is divided into three subclasses: receptor-regulated Smad's, activin/TGF alpha receptor-regulated (Smad2 and 3) or BMP receptor regulated (Smad1, 5, and 8); the common partner, (Smad4) that functions via its interaction to the various Smad's; and the inhibitory Smad's, (Smad6 and Smad7). Smad2 consists of two highly conserved domains, the N terminal Mad homology (MH1) and the C-terminal Mad homology 2 (MH2) domains. The MH1 domain binds DNA and regulates nuclear import and transcription while the MH2 domain conserved among all the Smad's regulates Smad2 oligomerization and binding to cytoplasmic adaptors and transcription factors. Activated Smad2 associates with Smad4 and translocates as a complex into the nucleus, allowing its binding to DNA and transcription factors. This translocation of Smad2 (as well as Smad3) into the nucleus is a central event in TGF beta signaling. Phosphorylation of threonine 8 in the calmodulin binding region of the MH1 domain by extracellular signal regulated kinase 1(ERK 1) enhances Smad2 transcriptional activity, which is negatively regulated by calmodulin. The regulation of Smad2 phosphorylation on threonine 8 by ERK 1 and calmodulin is critical for Smad2 mediated signaling.
Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
Momomer; the absence of TGF-beta. Heterodimer; in the presence of TGF-beta. Forms a heterodimer with co-SMAD, SMAD4, in the nucleus to form the transactivation complex SMAD2/SMAD4. Interacts with AIP1, HGS, PML and WWP1. Interacts with NEDD4L in response to TGF-beta. Found in a complex with SMAD3 and TRIM33 upon addition of TGF-beta. Interacts with ACVR1B, SMAD3 and TRIM33. Interacts (via the MH2 domain) with ZFYVE9; may form trimers with the SMAD4 co-SMAD. Interacts with FOXH1, homeobox protein TGIF, PEBP2-alpha subunit, CREB-binding protein (CBP), EP300 and SKI. Interacts with SNON; when phosphorylated at Ser-465/467. Interacts with SKOR1 and SKOR2. Interacts with PRDM16. Interacts (via MH2 domain) with LEMD3. Interacts with RBPMS. Interacts with WWP1. Interacts (dephosphorylated form, via the MH1 and MH2 domains) with RANBP3 (via its C-terminal R domain); the interaction results in the export of dephosphorylated SMAD3 out of the nucleus and termination ot the TGF-beta signaling. Interacts with PDPK1 (via PH domain).
Cytoplasm. Nucleus. Note=Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4. On dephosphorylation by phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1.
Expressed at high levels in skeletal muscle, heart and placenta.
Phosphorylated on one or several of Thr-220, Ser-245, Ser-250, and Ser-255. In response to TGF-beta, phosphorylated on Ser-465/467 by TGF-beta and activin type 1 receptor kinases. Able to interact with SMURF2 when phosphorylated on Ser-465/467, recruiting other proteins, such as SNON, for degradation. In response to decorin, the naturally occurring inhibitor of TGF-beta signaling, phosphorylated on Ser-240 by CaMK2. Phosphorylated by MAPK3 upon EGF stimulation; which increases transcriptional activity and stability, and is blocked by calmodulin. Phosphorylated by PDPK1.
In response to TGF-beta, ubiquitinated by NEDD4L; which promotes its degradation.
Acetylated on Lys-19 by coactivators in response to TGF-beta signaling, which increases transcriptional activity. Isoform short: Acetylation increases DNA binding activity in vitro and enhances its association with target promoters in vivo. Acetylation in the nucleus by EP300 is enhanced by TGF-beta.
Belongs to the dwarfin/SMAD family.
Contains 1 MH1 (MAD homology 1) domain.
Contains 1 MH2 (MAD homology 2) domain.
Entrez Gene: 4087 Human
Entrez Gene: 17126 Mouse
Entrez Gene: 29357 Rat
Omim: 601366 Human
SwissProt: Q15796 Human
SwissProt: Q62432 Mouse
SwissProt: O70436 Rat
Unigene: 12253 Human
Unigene: 705764 Human
Unigene: 391091 Mouse
Unigene: 2755 Rat
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications
SMAD2是参与细胞增殖、分化和发育的蛋白质家族的58 kDa成员。Smad家族被分为三个亚类：受体调节Smad、激活素/TGF-α受体调节（Smad2和3）或BMP受体调节（Smad1、5和8）；共同的伙伴（Smad4），其通过其与各种Smads的相互作用发挥作用；抑制Smad6，（Smad6和Sm）。AD7）。SMAD2由两个高度保守的结构域组成，N端MAD同源性（MH1）和C-末端MAD同源2（MH2）结构域。MH1结构域结合DNA并调节细胞核的导入和转录，而在所有SMAD中保守的MH2结构域调节SMAD2寡聚化并结合胞质转接器和转录因子。激活的Smad2与Smad4相关，并作为一个复合物转入细胞核，允许其与DNA和转录因子结合。SMAD2（以及SMAD3）在细胞核中的易位是TGF-β信号转导的中心事件。苏氨酸8在细胞外信号调节激酶1（ERK 1）的MH1结构域钙调素结合区中的***酸化增强SMAD2转录活性，其受钙调素的负调控。ERK 1和钙调素对苏氨酸8的SMAD2***酸化的调节是SMAD2介导的信号转导的关键。