Rabbit Anti-DEM1/PNK/FITC Conjugated antibody
|别 名||PNK1; Bifunctional polynucleotide phosphatase/kinase; DEM 1; DEM1; DNA 5' kinase/3' phosphatase; PNK 1; PNK; PNK1; PNKP; Polynucleotide 5' hydroxyl kinase; Polynucleotide kinase 3 prime phosphatase; Polynucleotide kinase 3' phosphatase; Polynucleotide Kinase; PNKP_HUMAN.|
|规格价格||100ul/2980元 购买 大包装/询价|
|说 明 书||100ul|
|研究领域||肿瘤 细胞生物 免疫学 信号转导 细胞凋亡 转录调节因子 激酶和***酸酶|
|交叉反应||Human, Mouse, Rat, Dog, Cow, Sheep,|
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
|分 子 量||57kDa|
|性 状||Lyophilized or Liquid|
|免 疫 原||KLH conjugated synthetic peptide derived from human PNK1/PNKP|
|纯化方法||affinity purified by Protein A|
|储 存 液||0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.|
|保存条件||Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.|
Mammalian PNK catalyzes the phosphorylation of DNA at 5'-hydroxyl termini and can dephosphorylate its 3'-phosphate termini. It plays an important function in DNA repair following ionizing radiation or oxidative damage. PNK has been reported to participate in the repair of DNA-double strand breaks via PARP-1-dependent nonhomologous end-joining.
Plays a key role in the repair of DNA damage,functioning as part of both the non-homologous end-joining (NHEJ)and base excision repair (BER) pathways. Through its two catalyticactivities, PNK ensures that DNA termini are compatible withextension and ligation by either removing 3'-phosphates from, or byphosphorylating 5'-hydroxyl groups on, the ribose sugar of the DNAbackbone.
Monomer (By similarity).
Expressed in many tissues with highestexpression in spleen and testis, and lowest expression in smallintestine (PubMed:10446192). Expressed in higher amount inpancreas, heart and kidney and at lower levels in brain, lung andliver (PubMed:10446193).
Phosphorylated upon DNA damage, probably by ATM or ATR.
Defects in PNKP are the cause of epilepticencephalopathy, early infantile, type 10 (EIEE10) [MIM:613402]. Adisease characterized by microcephaly, infantile-onset seizures,severe intellectual disability and delayed motor milestones withabsent speech or only achieving a few words. Most patients alsohave behavioral problems with hyperactivity. Microcephaly isprogressive and without neuronal migration or structuralabnormalities, consistent with primary microcephaly.
In the N-terminal section; belongs to the DNA 3'phosphatase family.
Contains 1 FHA domain.
Entrez Gene: 11284 Human
Omim: 605610 Human
SwissProt: Q96T60 Human
Unigene: 78016 Human
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications