Rabbit Anti-Phospho-PLK1 (Ser137)/FITC Conjugated antibody
|别 名||PLK1 (phospho S137); p-PLK1 (phospho S137); PLK1 (Phospho-Thr137); PLK1 (Phospho Thr137); p-PLK1 (Thr137); p-PLK1 (T137); Polo-Like Kinase(phospho T137); PLK 1; PLK; polio like kinase; Polo like kinase 1; Polo-like kinase 1; Serine/threonine protein kinase 13; Serine/threonine protein kinase PLK1; Serine/threonine-protein kinase; STPK 13; STPK13; Polo like kinase kinase; Cell cycle regulated protein kinase; PLK-1; plk1; PLK1_HUMAN.|
|规格价格||100ul/2980元 购买 大包装/询价|
|说 明 书||100ul|
|研究领域||肿瘤 细胞生物 免疫学 信号转导 转录调节因子 激酶和***酸酶|
|交叉反应||Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Rabbit,|
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
|分 子 量||68kDa|
|性 状||Lyophilized or Liquid|
|免 疫 原||KLH conjugated Synthesised phosphopeptide derived from human PLK1 around the phosphorylation site of Ser137|
|纯化方法||affinity purified by Protein A|
|储 存 液||0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.|
|保存条件||Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.|
PLK1 (polo-like kinase 1) is a member of the serine/threonine protein kinase family, cdc5/polo subfamily. PLK1 contains two polo box domains with a predicted molecular weight of 68 kDa. PLK1 has been shown to regulate cdc2/cyclin B through phosphorylation and activation of cdc25c phosphatase. PLK1 is modified by phosphorylation at Threonine 210. PLK1 may also be required for cell division. Depletion of PLK1 results in apoptosis and deregulation of expression of PKL1 is correlated with development of many malignancies.
Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis. Required for recovery after DNA damage checkpoint and entry into mitosis. Required for kinetochore localization of BUB1B. Phosphorylates SGOL1. Required for spindle pole localization of isoform 3 of SGOL1 and plays a role in regulating its centriole cohesion function. Phosphorylates BORA, and thereby promotes the degradation of BORA. Contributes to the regulation of AURKA function. Regulates TP53 stability through phosphorylation of TOPORS. Phosphorylates NEDD1. NEDD1 phosphorylation promotes subsequent targeting of the gamma-tubulin ring complex (gTuRC) to the centrosome, an important step for spindle formation. Phosphorylates both ECT2 and RACGAP1, and thereby stimulates their interaction that is essential for the cleavage furrow formation. Promotes the central spindle recruitment of ECT2.
Interacts with CEP170 and EVI5. Interacts and phosphorylates ERCC6L. Interacts with FAM29A. Interacts with SLX4/BTBD12 and TTDN1. Interacts with BUB1B. Interacts (via POLO-box domain) with the phosphorylated form of BUB1, MLF1IP and CDC25C. Interacts with isoform 3 of SGOL1. Interacts with BORA, KIF2A and AURKA. Interacts with TOPORS and CYLD. Interacts with ECT2; the interaction is stimulated upon phosphorylation of ECT2 on 'Thr-444'. Interacts with PRC1.
Nucleus. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, centrosome. Midbody. Note=During early stages of mitosis, the phosphorylated form is detected on centrosomes and kinetochores. Localizes to the outer kinetochore. Presence of SGOL1 and interaction with the phosphorylated form of BUB1 is required for the kinetochore localization.
Placenta and colon.
Catalytic activity is enhanced by phosphorylation of Thr-210. Phosphorylation at Thr-210 is first detected on centrosomes in the G2 phase of the cell cycle, peaks in prometaphase and gradually disappears from centrosomes during anaphase.
Autophosphorylation and phosphorylation of Ser-137 may not be significant for the activation of PLK1 during mitosis, but may enhance catalytic activity during recovery after DNA damage checkpoint.
Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) in anaphase and following DNA damage, leading to its degradation by the proteasome. Ubiquitination is mediated via its interaction with FZR1/CDH1. Ubiquitination and subsequent degradation prevents entry into mitosis and is essential to maintain an efficient G2 DNA damage checkpoint.
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.
Contains 2 POLO box domains.
Contains 1 protein kinase domain.
Entrez Gene: 5347 Human
Entrez Gene: 18817 Mouse
Entrez Gene: 25515 Rat
Omim: 602098 Human
SwissProt: P53350 Human
SwissProt: Q07832 Mouse
SwissProt: Q62673 Rat
Unigene: 592049 Human
Unigene: 16525 Mouse
Unigene: 11034 Rat
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications
PLK1（Polo样激酶1）是丝氨酸/苏氨酸蛋白激酶家族的成员，CDC5/Polo亚家族。PLK1包含两个预测分子量为68 kDa的Polo盒结构域。PLK1已被证明通过CDC25C***酸酶的***酸化和活化来调节CDC2/cyclin B。通过苏氨酸210的***酸化修饰PLK1。细胞分裂也需要PLK1。PLK1的缺失导致细胞凋亡和PKL1表达的下调与多种恶性肿瘤的发生发展有关。