Rabbit Anti-Phospho-VASP(Ser239)/FITC Conjugated antibody
|别 名||VASP(phospho S239); Vasodilator-stimulated phosphoprotein; Vasodilator-stimulated phosphoprotein; VASP; VASP_HUMAN.|
|规格价格||100ul/2980元 购买 大包装/询价|
|说 明 书||100ul|
|研究领域||肿瘤 免疫学 神经生物学 信号转导 细胞凋亡 转录调节因子 激酶和***酸酶|
|交叉反应||Human, Mouse, Rat, Dog, Cow,|
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
|分 子 量||40kDa|
|性 状||Lyophilized or Liquid|
|免 疫 原||KLH conjugated synthesised phosphopeptide derived from human VASP around the phosphorylation site of Ser239|
|纯化方法||affinity purified by Protein A|
|储 存 液||0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.|
|保存条件||Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.|
Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG. [provided by RefSeq, Jul 2008]
Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein. VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments. Plays a role in actin-based mobility of Listeria monocytogenes in host cells. Regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation.
Homotetramer. Interacts with PFN1, PFN2, LPP, ACTN1 and ACTG1. Interacts, via the EVH1 domain, with the Pro-rich regions of ZYX. This interaction is important for targeting to focal adhesions and the formation of actin-rich structures at the apical surface of cells. Interacts, via the EVH1 domain, with the Pro-rich domain of Listeria monocytogenes actA. Interacts with APBB1IP. Interacts, via the Pro-rich domain, with the C-terminal SH3 domain of DNMBP (By similarity).
Cytoplasm. Cytoplasm, cytoskeleton. Cell junction, focal adhesion. Cell projection, lamellipodium membrane. Cell projection, filopodium membrane. Note=Targeted to stress fibers and focal adhesions through interaction with a number of proteins including MRL family members. Localizes to the plasma membrane in protruding lamellipodia and filopodial tips. Stimulation by thrombin or PMA, also translocates VASP to focal adhesions. Localized along the sides of actin filaments throughout the peripheral cytoplasm under basal conditions.
Highly expressed in platelets.
Major substrate for cAMP-dependent (PKA) and cGMP-dependent protein kinase (PKG) in platelets. The preferred site for PKA is Ser-157, the preferred site for PKG/PRKG1, Ser-239. In ADP-activated platelets, phosphorylation by PKA or PKG on Ser-157 leads to fibrinogen receptor inhibition. Phosphorylation on Thr-278 requires prior phosphorylation on Ser-157 and Ser-239. In response to phorbol ester (PMA) stimulation, phosphorylated by PKC/PRKCA. In response to thrombin, phosphorylated by both PKC and ROCK1. Phosphorylation at Thr-278 by AMPK does not require prior phosphorylation at Ser-157 or Ser-239. Phosphorylation modulates F-actin binding, actin filament elongation and platelet activation. Phosphorylation at Ser-322 by AMPK also alters actin filament binding. Carbon monoxide (CO) promotes phosphorylation at Ser-157, while nitric oxide (NO) promotes phosphorylation at Ser-157, but also at Ser-239. Response to NO and CO is blunted in platelets from diabetic patients, and VASP is not phosphorylated efficiently at Ser-157 and Ser-239.
Belongs to the Ena/VASP family.
Contains 1 WH1 domain.
Entrez Gene: 403936 Dog
Entrez Gene: 7408 Human
Entrez Gene: 22323 Mouse
Entrez Gene: 361517 Rat
Omim: 601703 Human
SwissProt: P50551 Dog
SwissProt: P50552 Human
SwissProt: P70460 Mouse
Unigene: 515469 Human
Unigene: 9684 Mouse
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
VASP的***酸化受PKG（cGMP-dependent protein kinase）和PKA（cAMP—dependent protein kinase）的调控。在粘附斑的形成与脱落过程中，该***酸化起着一个“开关”的作用
血管舒张剂刺激***蛋白（Vasp）是ENA VASP蛋白家族的成员。ENA VASP家族成员包含EHV1 N-末端结构域，其结合含有E/DFPPPPXD／E基序的蛋白和靶向ENA VASP蛋白至局灶性粘连。在蛋白质的中间区域，家族成员具有富含SH3和WW结构域蛋白的脯氨酸富集结构域。它们的C-末端EVH2结构域介导四聚体并结合G和F肌动蛋白。VASP与丝状肌动蛋白的形成有关，并可能在细胞粘附和运动中起着广泛的作用。VASP也可能参与细胞内的信号通路调节整合素细胞外基质的相互作用。VASP的环核苷酸依赖性蛋白激酶PKA和PKG调节。[ RefSeq，JUL 2008 ]