Rabbit Anti-Phospho-SMC1(Ser957)/FITC Conjugated antibody
|别 名||SMC1(Phospho-Ser957); SMC1A(phospho S957); P-SMC1(Ser957);CDLS2; SMC1; Segregation of mitotic chromosomes 1; Segregation of mitotic chromosomes like 1; SMC 1; SMC1A; SMC1alpha; SMC1alpha protein; SMC1B; SMC1BETA; SMC1beta protein; SMC1L1; SMC1L2; SMCB; Structural maintenance of chromosome 1 like 1 protein; Structural maintenance of chromosome 1 like 2 protein; structural maintenance of chromosomes 1-like 2 (yeast); Structural maintenance of chromosomes 1A; Structural maintenance of chromosomes 1B; Structural Maintenance of Chromosomes-1 Like 1; SMC1A_HUMAN.|
|规格价格||100ul/2980元 购买 大包装/询价|
|说 明 书||100ul|
|研究领域||肿瘤 细胞生物 免疫学 染色质和核信号 转录调节因子|
|交叉反应||Human, Mouse, Sheep,|
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
|分 子 量||136kDa|
|性 状||Lyophilized or Liquid|
|免 疫 原||KLH conjugated Synthesised phosphopeptide derived from human SMC1 around the phosphorylation site of Ser957 [GS(p-S)QG]|
|纯化方法||affinity purified by Protein A|
|储 存 液||0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.|
|保存条件||Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.|
Structural Maintenance of Chromosomes (SMC) family proteins play critical roles in various nuclear events that require structural changes of chromosomes, including mitotic chromosome organization, DNA recombination and repair and global transcriptional repression. The chromosome proteins are conserved in eukaryotes and can lead to mitotic chromosome segregation defects, suggesting a critical function of SMC family proteins in mitotic chromosome dynamics. SMC1 and SMC3 form a heterodimeric complex required for metaphase progression in mitotic cells. Specifically this SMC1/SMC3 complex is responsible for sister chromatid cohesion during metaphase. A number of cellular factors interact with hSMC1/hSMC3 during cell cycle. The major population of hSMC1/hSMC3 is in a compex with hRAD21 forming the human cohesion complex. Human cohesion complex associates with chromosomes which peaks at S phase and dissociates from chromosomes during G2/M transition. In addition, a subpopulation of hSMC1/hSMC3 associates tightly with nuclear matrix and centrosomes during interphase. A subset of hSMC1/hSMC3 is localized to spindle poles, spindles and kinetochores during mitosis when cohesin is in the cytoplasm. hSMC1/hSMC3 is required for spindle aster formation in vitro and reacts with nuclear mitotic apparatus protein in vivo.
Involved in chromosome cohesion during cell cycle and in DNA repair. Central component of cohesin complex. The cohesion complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Involved in DNA repair via its interaction with BRCA1 and its related phosphorylation by ATM, or via its phosphorylation by ATR. Works as a downstream effector both in the ATM/NBS1 branch and in the ATR/MSH2 branch of S-phase checkpoint.
Interacts with POLE. Interacts with SYCP2. Interacts with BRCA1. Found in a complex with CDCA5, SMC3 and RAD21, PDS5A/APRIN and PDS5B/SCC-112 (By similarity). Forms a heterodimer with SMC3 in cohesin complexes. Cohesin complexes are composed of the SMC1 (SMC1A or SMC1B) and SMC3 heterodimer attached via their hinge domain, RAD21 which link them, and one STAG protein (STAG1, STAG2 or STAG3), which interacts with RAD21. In germ cell cohesion complexes, SMC1A is mutually exclusive with SMC1B. Interacts with BRCA1. Interacts with NDC80.
Nucleus. Chromosome. Chromosome, centromere, kinetochore. Note=Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesion complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesion complexes remain. At anaphase, the RAD21 subunit of the cohesion complex is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. In germ cells, cohesion complex dissociates from chromatin at prophase I, and may be replaced by a meiosis-specific cohesin complex. The phosphorylated form on Ser-957 and Ser-966 associates with chromatin during G1/S/G2 phases but not during M phase, suggesting that phosphorylation does not regulate cohesin function. Integral component of the functional centromere-kinetochore complex at the kinetochore region during mitosis
Phosphorylated by ATM upon ionizing radiation in a NBS1-dependent manner. Phosphorylated by ATR upon DNA methylation in a MSH2/MSH6-dependent manner. Phosphorylation of Ser-957 and Ser-966 activates it and is required for S-phase checkpoint activation.
Defects in SMC1A are the cause of Cornelia de Lange syndrome type 2 (CDLS2) [MIM:300590]; also known as Cornelia de Lange syndrome X-linked. CDLS is a clinically heterogeneous developmental disorder associated with malformations affecting multiple systems. CDLS is characterized by facial dysmorphisms, abnormal hands and feet, growth delay, cognitive retardation and various other malformations including gastroesophageal dysfunction and cardiac, ophthalmologic and genitourinary anomalies.
Belongs to the SMC family. SMC1 subfamily.
Entrez Gene: 27127 Human
Entrez Gene: 8243 Human
Entrez Gene: 140557 Mouse
Entrez Gene: 24061 Mouse
Entrez Gene: 300121 Rat
Entrez Gene: 63996 Rat
Omim: 300040 Human
Omim: 608685 Human
SwissProt: Q14683 Human
SwissProt: Q8NDV3 Human
SwissProt: Q920F6 Mouse
SwissProt: Q9CU62 Mouse
SwissProt: Q9Z1M9 Rat
Unigene: 334176 Human
Unigene: 182737 Mouse
Unigene: 56972 Rat
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications
染色体结构维修（SMC）家族蛋白在各种核事件需要染色体结构的变化起着至关重要的作用，包括有丝分裂染色体结构，DNA重组和修复和全球的转录抑制。染色体蛋白在真核生物中是保守的，可能会导致有丝分裂染色体分离的缺陷，表明SMC家族蛋白在有丝分裂染色体动力学的一个重要功能。SMC1和SMC3形成的异源二聚体，在细胞有丝分裂中期发展要求为。中期具体SMC1 / SMC3复杂负责姊妹染色单体的结合。一些细胞因子相互作用在细胞周期hsmc1 / hsmc3。对hsmc1 / hsmc3主要人口是一个复杂的复杂的人hRAD21形成凝聚力。人类凝聚的复杂结合的染色体，峰在S期和分裂的染色体在G2 / M期。此外，在相间的hsmc1 / hsmc3紧密联系与核基质与中心体的一个亚群。对hsmc1 / hsmc3子集定位于有丝分裂时纺锤体的两极，黏合在细胞质中的主轴和着丝粒。hsmc1 / hsmc3是体外主轴翠菊形成所需和核有丝分裂器蛋白在体内的反应。